Detailed information of TA system TA243345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1847678..1848477
• Replicon: chromosome
• Accession: NZ_CP096776
• Organism: Proteus mirabilis strain HURS-186083

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: M1766_RS08855
• Protein ID: WP_004247966.1
• Coordinates: 1847953..1848477 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: M1766_RS08850
• Protein ID: WP_004247964.1
• Coordinates: 1847678..1847956 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1766_RS08825 (M1766_08825)	1843028..1844110	+	1083	WP_004242680.1	peptide chain release factor 1	-
M1766_RS08830 (M1766_08830)	1844110..1844958	+	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
M1766_RS08835 (M1766_08835)	1844936..1845751	+	816	WP_004242688.1	invasion regulator SirB1	-
M1766_RS08840 (M1766_08840)	1845809..1846663	+	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
M1766_RS08845 (M1766_08845)	1846671..1847600	+	930	WP_017628160.1	TIGR01212 family radical SAM protein	-
M1766_RS08850 (M1766_08850)	1847678..1847956	+	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
M1766_RS08855 (M1766_08855)	1847953..1848477	+	525	WP_004247966.1	hypothetical protein	Toxin
M1766_RS08860 (M1766_08860)	1848558..1850258	-	1701	WP_004247967.1	C4-dicarboxylic acid transporter DauA	-
M1766_RS08870 (M1766_08870)	1851211..1852152	+	942	WP_036918236.1	glyoxylate/hydroxypyruvate reductase GhrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T243345 WP_004247966.1 NZ_CP096776:1847953-1848477 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T243345 NZ_OW970560:1624117-1624533 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT243345 WP_004247964.1 NZ_CP096776:1847678-1847956 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT243345 NZ_OW970560:1623890-1624120 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: B4EVS2