Detailed information of TA system TA243341

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3408727..3409748
• Replicon: chromosome
• Accession: NZ_CP096775
• Organism: Proteus mirabilis strain HURS-181823

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: M1765_RS16355
• Protein ID: WP_004249488.1
• Coordinates: 3409179..3409748 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: M1765_RS16350
• Protein ID: WP_004245528.1
• Coordinates: 3408727..3409179 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1765_RS16330 (M1765_16330)	3404513..3405043	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
M1765_RS16335 (M1765_16335)	3405360..3406325	+	966	WP_086935863.1	thiamine ABC transporter substrate binding subunit	-
M1765_RS16340 (M1765_16340)	3406325..3407905	+	1581	WP_046334723.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
M1765_RS16345 (M1765_16345)	3407898..3408599	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
M1765_RS16350 (M1765_16350)	3408727..3409179	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
M1765_RS16355 (M1765_16355)	3409179..3409748	+	570	WP_004249488.1	PIN domain-containing protein	Toxin
M1765_RS16360 (M1765_16360)	3409864..3412218	+	2355	WP_109828966.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21600.10 Da
• Isoelectric Point: 7.3470

>T243341 WP_004249488.1 NZ_CP096775:3409179-3409748 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKNPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T243341 NZ_OW970560:440225-440608 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT243341 WP_004245528.1 NZ_CP096775:3408727-3409179 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT243341 NZ_OW970560:439983-440225 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: B4F2H3