Detailed information of TA system TA243340

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1847685..1848484
• Replicon: chromosome
• Accession: NZ_CP096775
• Organism: Proteus mirabilis strain HURS-181823

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: M1765_RS08870
• Protein ID: WP_004247966.1
• Coordinates: 1847960..1848484 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: M1765_RS08865
• Protein ID: WP_004247964.1
• Coordinates: 1847685..1847963 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1765_RS08840 (M1765_08840)	1843035..1844117	+	1083	WP_004242680.1	peptide chain release factor 1	-
M1765_RS08845 (M1765_08845)	1844117..1844965	+	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
M1765_RS08850 (M1765_08850)	1844943..1845758	+	816	WP_004242688.1	invasion regulator SirB1	-
M1765_RS08855 (M1765_08855)	1845816..1846670	+	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
M1765_RS08860 (M1765_08860)	1846678..1847607	+	930	WP_017628160.1	TIGR01212 family radical SAM protein	-
M1765_RS08865 (M1765_08865)	1847685..1847963	+	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
M1765_RS08870 (M1765_08870)	1847960..1848484	+	525	WP_004247966.1	hypothetical protein	Toxin
M1765_RS08875 (M1765_08875)	1848565..1850265	-	1701	WP_004247967.1	C4-dicarboxylic acid transporter DauA	-
M1765_RS08885 (M1765_08885)	1851218..1852159	+	942	WP_036918236.1	glyoxylate/hydroxypyruvate reductase GhrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T243340 WP_004247966.1 NZ_CP096775:1847960-1848484 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T243340 NZ_OW970560:356941-357288 [Klebsiella pneumoniae]
ATGTGGGATGTGGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATAAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT243340 WP_004247964.1 NZ_CP096775:1847685-1847963 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT243340 NZ_OW970560:357288-357587 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: B4EVS2