Detailed information of TA system TA243323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3346100..3346782
• Replicon: chromosome
• Accession: NZ_CP096753
• Organism: Acinetobacter baumannii strain 5664

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: M1S46_RS16195
• Protein ID: WP_000009390.1
• Coordinates: 3346100..3346279 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1S2G1M8
• Locus tag: M1S46_RS16200
• Protein ID: WP_000878688.1
• Coordinates: 3346369..3346782 (+)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1S46_RS16175 (M1S46_16175)	3341896..3342756	-	861	WP_071211542.1	GPO family capsid scaffolding protein	-
M1S46_RS16180 (M1S46_16180)	3342934..3344712	+	1779	WP_071211543.1	terminase family protein	-
M1S46_RS16185 (M1S46_16185)	3344709..3345758	+	1050	WP_062936692.1	phage portal protein	-
M1S46_RS16190 (M1S46_16190)	3345768..3345983	+	216	WP_071211544.1	hypothetical protein	-
M1S46_RS16195 (M1S46_16195)	3346100..3346279	+	180	WP_000009390.1	type II toxin-antitoxin system HicA family toxin	Toxin
M1S46_RS16200 (M1S46_16200)	3346369..3346782	+	414	WP_000878688.1	hypothetical protein	Antitoxin
M1S46_RS16205 (M1S46_16205)	3347051..3347905	-	855	WP_071211545.1	SH3 domain-containing protein	-
M1S46_RS16230 (M1S46_16230)	3349036..3350100	-	1065	WP_001278262.1	quinolinate synthase NadA	-
M1S46_RS16235 (M1S46_16235)	3350247..3351467	-	1221	WP_000268130.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3308550..3346782	38232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-78)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6799.11 Da
• Isoelectric Point: 11.1422

>T243323 WP_000009390.1 NZ_CP096753:3346100-3346279 [Acinetobacter baumannii]
MSFNEFKRWLIAQGVIFVRKGKGSHMIIEFNGKKTVFPNHGKKEIPEGTRLKIKKDLGL

Nucleotide

• Length: 180 bp

>T243323 NZ_OW970541:c96999-96694 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15511.84 Da
• Isoelectric Point: 5.2905

>AT243323 WP_000878688.1 NZ_CP096753:3346369-3346782 [Acinetobacter baumannii]
MKYYVSIVQEEGAYIVSSRDLPLLNSVGYTLEEALSEALDGIETTFEIYMDERKAIPLPSKGKKDEYEIHLPVRVAAKVR
LYNEMISQDVTKAELARRLGWLQKQADRLLSLKHSTKLESIESAFHALGKDLDIVVA

Nucleotide

• Length: 414 bp

>AT243323 NZ_OW970541:c97217-96999 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1S2G1M8