243314

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 2497..3085 Replicon plasmid p5672
Accession NZ_CP096741
Organism Acinetobacter baumannii strain 5672

Toxin (Protein)


Gene name brnT Uniprot ID A0A0J8TIH5
Locus tag M1S23_RS19100 Protein ID WP_000438827.1
Coordinates 2497..2784 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID N9M5I3
Locus tag M1S23_RS19105 Protein ID WP_001983304.1
Coordinates 2771..3085 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1S23_RS19085 (M1S23_19085) 189..1124 + 936 WP_001292329.1 replication initiation protein RepM -
M1S23_RS19090 (M1S23_19090) 1202..1720 + 519 WP_000839337.1 plasmid replication DNA-binding protein -
M1S23_RS19095 (M1S23_19095) 1930..2229 + 300 WP_000358348.1 hypothetical protein -
M1S23_RS19100 (M1S23_19100) 2497..2784 + 288 WP_000438827.1 BrnT family toxin Toxin
M1S23_RS19105 (M1S23_19105) 2771..3085 + 315 WP_001983304.1 BrnA antitoxin family protein Antitoxin
M1S23_RS19110 (M1S23_19110) 3213..5624 + 2412 WP_000932953.1 TonB-dependent receptor ZnuD2 -
M1S23_RS19115 (M1S23_19115) 5929..6390 + 462 WP_000761346.1 DIP1984 family protein -
M1S23_RS19120 (M1S23_19120) 6716..6925 - 210 WP_000069474.1 hypothetical protein -
M1S23_RS19125 (M1S23_19125) 6918..7220 - 303 WP_001129974.1 XRE family transcriptional regulator -
M1S23_RS19130 (M1S23_19130) 7213..7569 - 357 WP_000269904.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..11196 11196


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11329.75 Da        Isoelectric Point: 5.6762

>T243314 WP_000438827.1 NZ_CP096741:2497-2784 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T243314 NZ_OW970540:1486791-1487207 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12208.84 Da        Isoelectric Point: 7.2026

>AT243314 WP_001983304.1 NZ_CP096741:2771-3085 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT243314 NZ_OW970540:1486564-1486794 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J8TIH5

Antitoxin

Source ID Structure
AlphaFold DB N9M5I3

References