Detailed information of TA system TA024330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3445531..3446156
• Replicon: chromosome
• Accession: NC_013361
• Organism: Escherichia coli O26:H11 str. 11368

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ECO26_RS18360
• Protein ID: WP_000911330.1
• Coordinates: 3445531..3445929 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: ECO26_RS18365
• Protein ID: WP_000450524.1
• Coordinates: 3445929..3446156 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO26_RS18340	3441409..3441609	+	201	WP_000383836.1	YpfN family protein	-
ECO26_RS18345	3441719..3442417	-	699	WP_000679812.1	esterase	-
ECO26_RS18350	3442491..3444506	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
ECO26_RS18355	3444521..3445384	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
ECO26_RS18360	3445531..3445929	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
ECO26_RS18365	3445929..3446156	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
ECO26_RS18370	3446310..3447023	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
ECO26_RS18380	3447236..3448270	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
ECO26_RS18385	3448287..3449165	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
ECO26_RS18390	3449311..3449883	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
ECO26_RS18395	3449883..3450353	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T24330 WP_000911330.1 NC_013361:c3445929-3445531 [Escherichia coli O26:H11 str. 11368]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T24330 NC_013361:c3445929-3445531 [Escherichia coli O26:H11 str. 11368]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT24330 WP_000450524.1 NC_013361:c3446156-3445929 [Escherichia coli O26:H11 str. 11368]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT24330 NC_013361:c3446156-3445929 [Escherichia coli O26:H11 str. 11368]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33