Detailed information of TA system TA243296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 30071..30686
• Replicon: plasmid p5745_1
• Accession: NZ_CP096715
• Organism: Acinetobacter baumannii strain 5745

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A0J8TIH5
• Locus tag: M1S38_RS19310
• Protein ID: WP_000438827.1
• Coordinates: 30399..30686 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: M1S38_RS19305
• Protein ID: WP_200165042.1
• Coordinates: 30071..30412 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1S38_RS19280 (M1S38_19280)	25617..25973	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	-
M1S38_RS19285 (M1S38_19285)	25966..26268	+	303	WP_001129974.1	XRE family transcriptional regulator	-
M1S38_RS19290 (M1S38_19290)	26261..26470	+	210	WP_000069474.1	hypothetical protein	-
M1S38_RS19295 (M1S38_19295)	26796..27256	-	461	Protein_32	DIP1984 family protein	-
M1S38_RS19300 (M1S38_19300)	27561..29971	-	2411	Protein_33	TonB-dependent receptor ZnuD2	-
M1S38_RS19305 (M1S38_19305)	30071..30412	-	342	WP_200165042.1	BrnA antitoxin family protein	Antitoxin
M1S38_RS19310 (M1S38_19310)	30399..30686	-	288	WP_000438827.1	BrnT family toxin	Toxin
M1S38_RS19315 (M1S38_19315)	30954..31253	-	300	WP_000358348.1	hypothetical protein	-
M1S38_RS19320 (M1S38_19320)	31629..32129	-	501	Protein_37	hypothetical protein	-
M1S38_RS19325 (M1S38_19325)	32433..33245	+	813	WP_249376159.1	MobA/MobL family protein	-
M1S38_RS19330 (M1S38_19330)	33427..33600	+	174	WP_228200477.1	hypothetical protein	-
M1S38_RS19335 (M1S38_19335)	34195..35129	+	935	Protein_40	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..44752	44752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11329.75 Da
• Isoelectric Point: 5.6762

>T243296 WP_000438827.1 NZ_CP096715:c30686-30399 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T243296 NZ_OW970517:c24418-24002 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCGTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAATTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13097.90 Da
• Isoelectric Point: 8.4751

>AT243296 WP_200165042.1 NZ_CP096715:c30412-30071 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKIINKNGSLGSHF

Nucleotide

• Length: 342 bp

>AT243296 NZ_OW970517:c24645-24415 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGCGAGGATGTGGTCAGCGACGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A0J8TIH5