Detailed information of TA system TA243239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 3675927..3677622
• Replicon: chromosome
• Accession: NZ_CP096665
• Organism: Pseudomonas aeruginosa strain PAO1_Mat-X-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: M0419_RS17555
• Protein ID: WP_003112901.1
• Coordinates: 3676753..3677622 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: M0419_RS17550
• Protein ID: WP_003112902.1
• Coordinates: 3675927..3676760 (-)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0419_RS17530 (M0419_17530)	3671158..3672642	-	1485	WP_003112906.1	aldehyde dehydrogenase	-
M0419_RS17535 (M0419_17535)	3672654..3673457	-	804	WP_003112905.1	aspartate dehydrogenase	-
M0419_RS17540 (M0419_17540)	3673468..3675150	-	1683	WP_003112904.1	thiamine pyrophosphate-binding protein	-
M0419_RS17545 (M0419_17545)	3675143..3675940	-	798	WP_003112903.1	SDR family oxidoreductase	-
M0419_RS17550 (M0419_17550)	3675927..3676760	-	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
M0419_RS17555 (M0419_17555)	3676753..3677622	-	870	WP_003112901.1	alpha/beta hydrolase	Toxin
M0419_RS17560 (M0419_17560)	3677631..3678161	-	531	WP_003112900.1	cupin domain-containing protein	-
M0419_RS17565 (M0419_17565)	3678175..3678936	-	762	WP_003112899.1	SDR family oxidoreductase	-
M0419_RS17570 (M0419_17570)	3678942..3679718	-	777	WP_003112898.1	ABC transporter permease	-
M0419_RS17575 (M0419_17575)	3679715..3680722	-	1008	WP_003112897.1	ABC transporter substrate-binding protein	-
M0419_RS17580 (M0419_17580)	3680763..3681614	-	852	WP_003130104.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T243239 WP_003112901.1 NZ_CP096665:c3677622-3676753 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T243239 NZ_OW970452:c4742263-4741979 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT243239 WP_003112902.1 NZ_CP096665:c3676760-3675927 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT243239 NZ_OW970452:c4742495-4742253 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1