Detailed information of TA system TA243229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 1115960..1117218
• Replicon: chromosome
• Accession: NZ_CP096664
• Organism: Pseudomonas aeruginosa strain PAO1_Kat-X-2

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: M0418_RS05335
• Protein ID: WP_003112528.1
• Coordinates: 1116463..1117218 (+)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: M0418_RS05330
• Protein ID: WP_003086329.1
• Coordinates: 1115960..1116328 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0418_RS05310 (M0418_05310)	1112123..1112398	+	276	WP_010793902.1	zinc ribbon domain-containing protein	-
M0418_RS05315 (M0418_05315)	1112401..1112877	-	477	WP_003112531.1	hypothetical protein	-
M0418_RS05320 (M0418_05320)	1113052..1114545	+	1494	WP_003112530.1	aldehyde dehydrogenase family protein	-
M0418_RS05325 (M0418_05325)	1114601..1115887	+	1287	WP_003112529.1	FAD-binding oxidoreductase	-
M0418_RS05330 (M0418_05330)	1115960..1116328	+	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
M0418_RS05335 (M0418_05335)	1116463..1117218	+	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
M0418_RS05340 (M0418_05340)	1117986..1119347	+	1362	WP_010895536.1	DNA recombination protein RmuC	-
M0418_RS05345 (M0418_05345)	1119502..1122045	-	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T243229 WP_003112528.1 NZ_CP096664:1116463-1117218 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T243229 NZ_OW970451:2484-2822 [Klebsiella pneumoniae]
GTGACGACATATTGTCCAGCCAGAGGCGATGTCATTCTTTTAGATTTTAACCCTCAATCAGGGCATGAGCAGGCAGGGAA
AAGGCCTGCTCTTGTCGTTTCTGATGATTTGTTTAACCAAGTCACTGGGTTTGCTGTTGTTTGCCCCATAACTAATCAGA
TAAAAGGGTACCCTTTTGAAGTTCCTGTTGATGGGACTACTAAAACAACAGGGGTTATCTTGGCTGATCAAGTGAAGTCT
TTAGACTGGAAGGCCAGAGCGGCACGTACTGTTGATAGCGTTTCCGGCGAGACAGTAACTACAGTTGTTGATATGGTTTC
AAAAATTATTAAAGCATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT243229 WP_003086329.1 NZ_CP096664:1115960-1116328 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT243229 NZ_OW970451:2257-2487 [Klebsiella pneumoniae]
ATGACTACGGCTATTGGTAAATGGGGTAATGGCGCTGCTTTGCGTATCCCGCAGCCGTTCATGAAGCAGCTTGGGCTTAG
TATCGGTGATGTTGTTAATTTCCGCGTCACTGAAAGGGATTTAGTAATTACTAAAGCTGGCCCGACGCTTGAAGAACTGC
TCGGACAGTGCACTGCAGAAAATCGCCATCACGAGTATCTATGTGACTCTCAAGGTAAGGAGATGCTGTGA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7