Detailed information of TA system TA243228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 796843..797451
• Replicon: chromosome
• Accession: NZ_CP096664
• Organism: Pseudomonas aeruginosa strain PAO1_Kat-X-2

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: Q9I5J9
• Locus tag: M0418_RS03745
• Protein ID: WP_003114156.1
• Coordinates: 797104..797451 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: M0418_RS03740
• Protein ID: WP_003114155.1
• Coordinates: 796843..797094 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0418_RS03720 (M0418_03720)	792490..792846	+	357	WP_003114150.1	DUF2523 family protein	-
M0418_RS03725 (M0418_03725)	792850..794124	+	1275	WP_025769036.1	zonular occludens toxin family protein	-
M0418_RS03730 (M0418_03730)	794354..795646	+	1293	WP_252957672.1	hypothetical protein	-
M0418_RS03735 (M0418_03735)	795646..796629	+	984	WP_003114154.1	tyrosine-type recombinase/integrase	-
M0418_RS03740 (M0418_03740)	796843..797094	+	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
M0418_RS03745 (M0418_03745)	797104..797451	+	348	WP_003114156.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M0418_RS03755 (M0418_03755)	797778..798680	-	903	WP_003114157.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
M0418_RS03760 (M0418_03760)	799132..799869	-	738	WP_003114158.1	murein L,D-transpeptidase catalytic domain family protein	-
M0418_RS03765 (M0418_03765)	799949..800992	-	1044	WP_003098363.1	L,D-transpeptidase	-
M0418_RS03770 (M0418_03770)	801128..801820	-	693	WP_003114159.1	16S rRNA pseudouridine(516) synthase	-
M0418_RS03775 (M0418_03775)	801820..802032	-	213	WP_003114160.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	775360..806555	31195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12984.78 Da
• Isoelectric Point: 4.4212

>T243228 WP_003114156.1 NZ_CP096664:797104-797451 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T243228 NZ_OW970447:17688-18104 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT243228 WP_003114155.1 NZ_CP096664:796843-797094 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT243228 NZ_OW970447:17461-17691 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q9I5J9

Antitoxin

• AlphaFold DB: A0A0B0C355