Detailed information of TA system TA243227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 143567..144072
• Replicon: chromosome
• Accession: NZ_CP096664
• Organism: Pseudomonas aeruginosa strain PAO1_Kat-X-2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: M0418_RS00660
• Protein ID: WP_003083773.1
• Coordinates: 143567..143848 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9I707
• Locus tag: M0418_RS00665
• Protein ID: WP_003112628.1
• Coordinates: 143845..144072 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0418_RS00635 (M0418_00635)	138818..140167	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
M0418_RS00640 (M0418_00640)	140216..140902	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
M0418_RS00645 (M0418_00645)	141003..141737	+	735	WP_003115036.1	GntR family transcriptional regulator	-
M0418_RS00650 (M0418_00650)	141917..142327	+	411	WP_003101225.1	aegerolysin family protein	-
M0418_RS00655 (M0418_00655)	142359..143267	-	909	WP_003083769.1	LysR family transcriptional regulator	-
M0418_RS00660 (M0418_00660)	143567..143848	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
M0418_RS00665 (M0418_00665)	143845..144072	-	228	WP_003112628.1	CopG family ribbon-helix-helix protein	Antitoxin
M0418_RS00670 (M0418_00670)	144248..144868	-	621	WP_003101226.1	hypothetical protein	-
M0418_RS00675 (M0418_00675)	144969..145469	+	501	WP_003112629.1	LEA type 2 family protein	-
M0418_RS00680 (M0418_00680)	145542..145883	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
M0418_RS00685 (M0418_00685)	145965..147392	-	1428	WP_003083784.1	GABA permease	-
M0418_RS00690 (M0418_00690)	147561..149054	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T243227 WP_003083773.1 NZ_CP096664:c143848-143567 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T243227 NZ_OW970446:49647-49958 [Klebsiella pneumoniae]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8568.64 Da
• Isoelectric Point: 5.5740

>AT243227 WP_003112628.1 NZ_CP096664:c144072-143845 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHIEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT243227 NZ_OW970446:49955-50374 [Klebsiella pneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• PDB: 6XRW
• AlphaFold DB: Q9I707