Detailed information of TA system TA243224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 967066..967595
• Replicon: chromosome
• Accession: NZ_CP096594
• Organism: Paracoccus denitrificans strain DYTN-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: M0K93_RS19585
• Protein ID: WP_221246478.1
• Coordinates: 967317..967595 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A0V0Q4K1
• Locus tag: M0K93_RS19580
• Protein ID: WP_010396097.1
• Coordinates: 967066..967311 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0K93_RS19550 (M0K93_19555)	962321..962773	-	453	WP_011750059.1	CoA-binding protein	-
M0K93_RS19555 (M0K93_19560)	962957..963346	-	390	WP_231000582.1	YHS domain-containing (seleno)protein	-
M0K93_RS19560 (M0K93_19565)	963471..964268	-	798	WP_011750061.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
M0K93_RS19570 (M0K93_19575)	964750..965607	+	858	WP_011750063.1	hypothetical protein	-
M0K93_RS19575 (M0K93_19580)	965600..966961	+	1362	WP_011750064.1	plasmid recombination protein	-
M0K93_RS19580 (M0K93_19585)	967066..967311	+	246	WP_010396097.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
M0K93_RS19585 (M0K93_19590)	967317..967595	+	279	WP_221246478.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M0K93_RS19590 (M0K93_19595)	967654..968187	+	534	WP_041530416.1	hypothetical protein	-
M0K93_RS19595 (M0K93_19600)	968412..969584	-	1173	WP_011750067.1	ATP-binding protein	-
M0K93_RS19600 (M0K93_19605)	969912..970121	-	210	WP_011748680.1	hypothetical protein	-
M0K93_RS19605 (M0K93_19610)	970263..970523	-	261	WP_221246477.1	hypothetical protein	-
M0K93_RS19610 (M0K93_19615)	970538..971047	-	510	WP_011750069.1	hypothetical protein	-
M0K93_RS19615 (M0K93_19620)	971044..971226	-	183	WP_028717528.1	hypothetical protein	-
M0K93_RS19620 (M0K93_19625)	971223..971522	-	300	WP_232290539.1	zinc ribbon domain-containing protein	-
M0K93_RS19625 (M0K93_19630)	971656..971901	+	246	WP_011750071.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	964497..983220	18723

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Antitoxin ParD (1-76)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10487.84 Da
• Isoelectric Point: 5.5730

>T243224 WP_221246478.1 NZ_CP096594:967317-967595 [Paracoccus denitrificans]
MAFTVAATVDLEDIWDYTEGEWGLAQARRYAEELRDTCHALASGERRGRKVDVRPGYLKCLSGSHIIFFRDLGDTLEIVR
ILHGAQDVERHL

Nucleotide

• Length: 279 bp

>T243224 NZ_OW970444:c5004220-5003303 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8708.75 Da
• Isoelectric Point: 6.4631

>AT243224 WP_010396097.1 NZ_CP096594:967066-967311 [Paracoccus denitrificans]
MSRNTSVSLGDHFVSFIDAQVKGGRYGSASDVVRAGLRLLEEHEAKVKALQDALIAGEDSGRAEGFDLDQFIARKMAGRN
A

Nucleotide

• Length: 246 bp

>AT243224 NZ_OW970444:c5004984-5004226 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGAGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0V0Q4K1