Detailed information of TA system TA243222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 357641..358326
• Replicon: chromosome
• Accession: NZ_CP096594
• Organism: Paracoccus denitrificans strain DYTN-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A1B6X9
• Locus tag: M0K93_RS16300
• Protein ID: WP_011749459.1
• Coordinates: 357641..358066 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A1B6Y0
• Locus tag: M0K93_RS16305
• Protein ID: WP_011749460.1
• Coordinates: 358063..358326 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0K93_RS16285 (M0K93_16290)	354315..356324	+	2010	WP_011749455.1	conjugal transfer protein TraG	-
M0K93_RS16290 (M0K93_16295)	356355..356750	+	396	Protein_355	CopG family transcriptional regulator	-
M0K93_RS16295 (M0K93_16300)	356902..357444	-	543	WP_041530330.1	AAA family ATPase	-
M0K93_RS16300 (M0K93_16305)	357641..358066	-	426	WP_011749459.1	type II toxin-antitoxin system VapC family toxin	Toxin
M0K93_RS16305 (M0K93_16310)	358063..358326	-	264	WP_011749460.1	plasmid stabilization protein	Antitoxin
M0K93_RS16310 (M0K93_16315)	358606..359610	+	1005	WP_011749461.1	P-type conjugative transfer ATPase TrbB	-
M0K93_RS16315 (M0K93_16320)	359607..359939	+	333	WP_011749462.1	TrbC/VirB2 family protein	-
M0K93_RS16320 (M0K93_16325)	359939..360220	+	282	WP_011749463.1	VirB3 family type IV secretion system protein	-
M0K93_RS16325 (M0K93_16330)	360233..362866	+	2634	WP_011749464.1	conjugal transfer protein TrbE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aac(3)-Ih	-	180731..360724	179993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15061.32 Da
• Isoelectric Point: 4.7222

>T243222 WP_011749459.1 NZ_CP096594:c358066-357641 [Paracoccus denitrificans]
MILLDTNVVSEPMREAPNPGVIGWIDDQAIETLHLSAITVAEVRFGIAVLPDGKRKARLQSRFETEILPLFADRILPFDL
AASEAFAALMAHARDKSAAIGKADGYIAAIAAAHRLTVATRDTAPFLAAGVTVIDPWDEAG

Nucleotide

• Length: 426 bp

>T243222 NZ_OW970444:4114187-4114405 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9852.22 Da
• Isoelectric Point: 10.6440

>AT243222 WP_011749460.1 NZ_CP096594:c358326-358063 [Paracoccus denitrificans]
MPAITIRNLSAETHRALKMRAAQHGRSTEAEVRDILEQAARPQHRVRLGSLLAEIGREARMTKEEHALFETAIRRDRAPA
QPIDFGE

Nucleotide

• Length: 264 bp

>AT243222 NZ_OW970444:4113786-4114160 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A1B6X9

Antitoxin

• AlphaFold DB: A1B6Y0