Detailed information of TA system TA243221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2213498..2214168
• Replicon: chromosome
• Accession: NZ_CP096593
• Organism: Paracoccus denitrificans strain DYTN-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A5P8JDY4
• Locus tag: M0K93_RS11325
• Protein ID: WP_011748362.1
• Coordinates: 2213734..2214168 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A1B3S2
• Locus tag: M0K93_RS11320
• Protein ID: WP_011748361.1
• Coordinates: 2213498..2213737 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0K93_RS11285 (M0K93_11290)	2208684..2210045	-	1362	WP_049792366.1	ParB N-terminal domain-containing protein	-
M0K93_RS11290 (M0K93_11295)	2210174..2210569	-	396	WP_011748356.1	HNH endonuclease signature motif containing protein	-
M0K93_RS11295 (M0K93_11300)	2210657..2211034	-	378	WP_011748357.1	DUF6362 family protein	-
M0K93_RS11300 (M0K93_11305)	2211027..2211251	-	225	WP_232290453.1	hypothetical protein	-
M0K93_RS11305 (M0K93_11310)	2211572..2212153	-	582	WP_049792326.1	hypothetical protein	-
M0K93_RS11310 (M0K93_11315)	2212584..2213222	+	639	WP_049792327.1	hypothetical protein	-
M0K93_RS11315 (M0K93_11320)	2213174..2213374	-	201	WP_011748360.1	hypothetical protein	-
M0K93_RS11320 (M0K93_11325)	2213498..2213737	+	240	WP_011748361.1	Arc family DNA-binding protein	Antitoxin
M0K93_RS11325 (M0K93_11330)	2213734..2214168	+	435	WP_011748362.1	type II toxin-antitoxin system VapC family toxin	Toxin
M0K93_RS11330 (M0K93_11335)	2214156..2214872	-	717	WP_011748363.1	sigma-70 family RNA polymerase sigma factor	-
M0K93_RS11335 (M0K93_11340)	2215297..2217459	-	2163	WP_011748365.1	hypothetical protein	-
M0K93_RS11340 (M0K93_11345)	2217793..2218248	+	456	WP_011748367.1	DUF2924 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2204207..2219573	15366

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

• Arc-like DNA binding domain (3-42)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15536.75 Da
• Isoelectric Point: 4.5849

>T243221 WP_011748362.1 NZ_CP096593:2213734-2214168 [Paracoccus denitrificans]
MIILDTNVVSETMKPAPEPRVIDWLNRQELTTLHLTTISLAELRFGIACLDAGRRRDDLDARLEQMLAEVFPARILSFDE
AAASAFGVLMATARRQGQAVSFADGAIAAIAAAQGYPVASRDTAPFAAMGVEVVDPWHEAAQGR

Nucleotide

• Length: 435 bp

>T243221 NZ_OW970444:c3937058-3936741 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8685.85 Da
• Isoelectric Point: 7.2058

>AT243221 WP_011748361.1 NZ_CP096593:2213498-2213737 [Paracoccus denitrificans]
MAQIVVRQIPEEVHRALKAQAAAHGRSAEAELREIIARAVLPQSRPRAGDLMRSIWSGAETDDLSVERDRTPAKPAGFE

Nucleotide

• Length: 240 bp

>AT243221 NZ_OW970444:c3936748-3936461 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A5P8JDY4

Antitoxin

• AlphaFold DB: A1B3S2