Detailed information of TA system TA243219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1457575..1458491
• Replicon: chromosome
• Accession: NZ_CP096592
• Organism: Bacillus inaquosorum strain A65.1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: M1M80_RS07170
• Protein ID: WP_060398450.1
• Coordinates: 1457745..1458491 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4NV07
• Locus tag: M1M80_RS07165
• Protein ID: WP_003239095.1
• Coordinates: 1457575..1457745 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1M80_RS07125 (M1M80_07125)	1452655..1454424	+	1770	WP_248596976.1	terminase	-
M1M80_RS07130 (M1M80_07130)	1454436..1454765	+	330	WP_060398446.1	XkdW family protein	-
M1M80_RS07135 (M1M80_07135)	1454762..1454926	+	165	WP_019258164.1	XkdX family protein	-
M1M80_RS07140 (M1M80_07140)	1454973..1455812	+	840	WP_060398447.1	hypothetical protein	-
M1M80_RS07145 (M1M80_07145)	1455865..1456134	+	270	WP_060398448.1	hemolysin XhlA family protein	-
M1M80_RS07150 (M1M80_07150)	1456147..1456410	+	264	WP_003239099.1	phage holin	-
M1M80_RS07155 (M1M80_07155)	1456423..1457316	+	894	WP_060398449.1	N-acetylmuramoyl-L-alanine amidase	-
M1M80_RS07160 (M1M80_07160)	1457353..1457490	-	138	WP_119913750.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
M1M80_RS07165 (M1M80_07165)	1457575..1457745	-	171	WP_003239095.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
M1M80_RS07170 (M1M80_07170)	1457745..1458491	-	747	WP_060398450.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
M1M80_RS07175 (M1M80_07175)	1458601..1459602	-	1002	WP_003239093.1	inorganic phosphate transporter	-
M1M80_RS07180 (M1M80_07180)	1459615..1460232	-	618	WP_003218470.1	DUF47 domain-containing protein	-
M1M80_RS07185 (M1M80_07185)	1460508..1461824	-	1317	WP_060398451.1	serine/threonine exchanger	-
M1M80_RS07190 (M1M80_07190)	1462210..1463160	+	951	WP_060398452.1	ring-cleaving dioxygenase	-
M1M80_RS07195 (M1M80_07195)	1463261..1463406	+	146	Protein_1353	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1424953..1524862	99909

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29097.57 Da
• Isoelectric Point: 4.6191

>T243219 WP_060398450.1 NZ_CP096592:c1458491-1457745 [Bacillus inaquosorum]
MLLFFQIMVWCVMAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPSSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQNRLDRKDVYYDQYGKMVLIPFTIETQSYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T243219 NZ_OW970444:2045876-2045978 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6555.59 Da
• Isoelectric Point: 10.8401

>AT243219 WP_003239095.1 NZ_CP096592:c1457745-1457575 [Bacillus inaquosorum]
MERAFQNGCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT243219 NZ_OW970444:c2045985-2045840 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Antitoxin

• AlphaFold DB: G4NV07