Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 43530..45153 Replicon plasmid pEC6622-1
Accession NZ_CP096588
Organism Escherichia coli strain EC6622

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag M1B75_RS23390 Protein ID WP_000088046.1
Coordinates 43530..44855 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag M1B75_RS23395 Protein ID WP_024136327.1
Coordinates 44860..45153 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1B75_RS23355 (M1B75_23355) 39422..40435 + 1014 WP_000845048.1 class 1 integron integrase IntI1 -
M1B75_RS23360 (M1B75_23360) 40638..40988 + 351 WP_000454193.1 DUF3330 domain-containing protein -
M1B75_RS23365 (M1B75_23365) 41114..41689 + 576 WP_072740834.1 recombinase family protein -
M1B75_RS23370 (M1B75_23370) 41736..42440 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
M1B75_RS23375 (M1B75_23375) 42493..42738 - 246 Protein_50 HNH endonuclease -
M1B75_RS23380 (M1B75_23380) 42751..43161 - 411 WP_000985911.1 hypothetical protein -
M1B75_RS23385 (M1B75_23385) 43263..43469 - 207 WP_000134171.1 hypothetical protein -
M1B75_RS23390 (M1B75_23390) 43530..44855 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
M1B75_RS23395 (M1B75_23395) 44860..45153 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
M1B75_RS23400 (M1B75_23400) 45757..46149 - 393 WP_000341066.1 hypothetical protein -
M1B75_RS23405 (M1B75_23405) 46522..46863 + 342 WP_001151575.1 hypothetical protein -
M1B75_RS23410 (M1B75_23410) 46955..47146 + 192 WP_000398480.1 hypothetical protein -
M1B75_RS23415 (M1B75_23415) 47196..47477 + 282 WP_000077926.1 hypothetical protein -
M1B75_RS25430 47818..47949 - 132 WP_000374059.1 hypothetical protein -
M1B75_RS23420 (M1B75_23420) 48040..49281 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / blaNDM-5 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aph(4)-Ia / aac(3)-IVa / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / floR / dfrA14 / blaOXA-10 / ARR-3 / qnrS1 / tet(A) - 1..262413 262413


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T243205 WP_000088046.1 NZ_CP096588:c44855-43530 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T243205 NZ_OW970436:836208-836618 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT243205 WP_024136327.1 NZ_CP096588:c45153-44860 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT243205 NZ_OW970436:835961-836227 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References