Detailed information of TA system TA243200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3544100..3544937
• Replicon: chromosome
• Accession: NZ_CP096587
• Organism: Escherichia coli strain EC6622

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: M1B75_RS17350
• Protein ID: WP_063103936.1
• Coordinates: 3544395..3544937 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: M1B75_RS17345
• Protein ID: WP_001297137.1
• Coordinates: 3544100..3544411 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1B75_RS17320 (3539120)	3539120..3540067	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
M1B75_RS17325 (3540089)	3540089..3542080	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
M1B75_RS17330 (3542070)	3542070..3542684	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
M1B75_RS17335 (3542684)	3542684..3543013	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
M1B75_RS17340 (3543025)	3543025..3543915	+	891	WP_000971336.1	heme o synthase	-
M1B75_RS17345 (3544100)	3544100..3544411	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
M1B75_RS17350 (3544395)	3544395..3544937	+	543	WP_063103936.1	GNAT family N-acetyltransferase	Toxin
M1B75_RS17355 (3544993)	3544993..3545928	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
M1B75_RS17360 (3546336)	3546336..3547700	+	1365	WP_001000978.1	MFS transporter	-
M1B75_RS17365 (3547828)	3547828..3548319	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
M1B75_RS17370 (3548487)	3548487..3549398	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19835.05 Da
• Isoelectric Point: 8.3395

>T243200 WP_063103936.1 NZ_CP096587:3544395-3544937 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILTA

Nucleotide

• Length: 543 bp

>T243200 NZ_OW970422:c4912239-4911364 [Klebsiella pneumoniae]
ATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATTTGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGA
TTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGGTGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGG
ATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAGGCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAG
ATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAAGATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACC
ACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTTTTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCT
ACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCATCCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAG
GAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTATTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCC
TCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAATTCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGA
CAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCCTGTATCACACAACAGGATGTCGATTTTTTACTGTCATTTAAA
CGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCGTATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATAT
TCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAGATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT243200 WP_001297137.1 NZ_CP096587:3544100-3544411 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT243200 NZ_OW970422:c4913045-4912287 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3