Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3510023..3510641 Replicon chromosome
Accession NZ_CP096587
Organism Escherichia coli strain EC6622

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag M1B75_RS17180 Protein ID WP_001291435.1
Coordinates 3510423..3510641 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag M1B75_RS17175 Protein ID WP_000344800.1
Coordinates 3510023..3510397 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1B75_RS17165 (3505112) 3505112..3506305 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
M1B75_RS17170 (3506328) 3506328..3509477 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
M1B75_RS17175 (3510023) 3510023..3510397 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
M1B75_RS17180 (3510423) 3510423..3510641 + 219 WP_001291435.1 HHA domain-containing protein Toxin
M1B75_RS17185 (3510813) 3510813..3511364 + 552 WP_000102568.1 maltose O-acetyltransferase -
M1B75_RS17190 (3511480) 3511480..3511950 + 471 WP_000136192.1 YlaC family protein -
M1B75_RS17195 (3512114) 3512114..3513664 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
M1B75_RS17200 (3513706) 3513706..3514059 - 354 WP_000878140.1 DUF1428 domain-containing protein -
M1B75_RS17210 (3514438) 3514438..3514749 + 312 WP_000409911.1 MGMT family protein -
M1B75_RS17215 (3514780) 3514780..3515352 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T243199 WP_001291435.1 NZ_CP096587:3510423-3510641 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T243199 NZ_OW970422:c4751909-4751625 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT243199 WP_000344800.1 NZ_CP096587:3510023-3510397 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT243199 NZ_OW970422:c4752141-4751899 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References