Detailed information of TA system TA243197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2480193..2480831
• Replicon: chromosome
• Accession: NZ_CP096587
• Organism: Escherichia coli strain EC6622

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: M1B75_RS12180
• Protein ID: WP_000813794.1
• Coordinates: 2480655..2480831 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: M1B75_RS12175
• Protein ID: WP_001270286.1
• Coordinates: 2480193..2480609 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1B75_RS12155 (2475345)	2475345..2476286	-	942	WP_001251319.1	ABC transporter permease	-
M1B75_RS12160 (2476287)	2476287..2477300	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
M1B75_RS12165 (2477318)	2477318..2478463	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
M1B75_RS12170 (2478708)	2478708..2480114	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
M1B75_RS12175 (2480193)	2480193..2480609	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
M1B75_RS12180 (2480655)	2480655..2480831	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
M1B75_RS12185 (2481053)	2481053..2481283	+	231	WP_000494244.1	YncJ family protein	-
M1B75_RS12190 (2481375)	2481375..2483336	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
M1B75_RS12195 (2483409)	2483409..2483945	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
M1B75_RS12200 (2484037)	2484037..2485212	+	1176	WP_001236319.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2485252..2486517	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T243197 WP_000813794.1 NZ_CP096587:c2480831-2480655 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T243197 NZ_OW970422:4033518-4033736 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT243197 WP_001270286.1 NZ_CP096587:c2480609-2480193 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT243197 NZ_OW970422:4033117-4033491 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• PDB: 6HPB