Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2574524..2574995 Replicon chromosome
Accession NC_013361
Organism Escherichia coli O26:H11 str. 11368

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A1Y2Y8G6
Locus tag ECO26_RS13875 Protein ID WP_001448501.1
Coordinates 2574717..2574995 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag ECO26_RS13870 Protein ID WP_001302048.1
Coordinates 2574524..2574715 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO26_RS13820 2569602..2569820 - 219 WP_000683609.1 DUF4014 family protein -
ECO26_RS13825 2569948..2570259 - 312 WP_001289673.1 hypothetical protein -
ECO26_RS13830 2570252..2570479 - 228 WP_000699809.1 hypothetical protein -
ECO26_RS13835 2570476..2570757 - 282 WP_000603384.1 DNA-binding protein -
ECO26_RS13840 2570790..2571551 - 762 WP_000450872.1 DUF1627 domain-containing protein -
ECO26_RS13845 2571577..2572318 - 742 Protein_2645 ATP-binding protein -
ECO26_RS13850 2572325..2573287 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
ECO26_RS13855 2573310..2573735 - 426 WP_000693943.1 Rha family transcriptional regulator -
ECO26_RS13860 2573732..2574034 - 303 WP_000172738.1 transcriptional regulator -
ECO26_RS32140 2574132..2574503 + 372 WP_001169687.1 hypothetical protein -
ECO26_RS13870 2574524..2574715 + 192 WP_001302048.1 hypothetical protein Antitoxin
ECO26_RS13875 2574717..2574995 + 279 WP_001448501.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECO26_RS13880 2575282..2575434 + 153 WP_001427316.1 DUF1391 family protein -
ECO26_RS13885 2575855..2576076 + 222 WP_000560218.1 killing protein KilR -
ECO26_RS13890 2576070..2576246 + 177 WP_000245534.1 hypothetical protein -
ECO26_RS13895 2576320..2576595 + 276 WP_001307773.1 hypothetical protein -
ECO26_RS13900 2576694..2579345 + 2652 WP_000105101.1 exodeoxyribonuclease VIII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 2524506..2587875 63369


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10691.27 Da        Isoelectric Point: 5.5647

>T24319 WP_001448501.1 NC_013361:2574717-2574995 [Escherichia coli O26:H11 str. 11368]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T24319 NC_013361:2574717-2574995 [Escherichia coli O26:H11 str. 11368]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTAGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT24319 WP_001302048.1 NC_013361:2574524-2574715 [Escherichia coli O26:H11 str. 11368]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT24319 NC_013361:2574524-2574715 [Escherichia coli O26:H11 str. 11368]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y2Y8G6

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References