Detailed information of TA system TA243186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1315638..1316263
• Replicon: chromosome
• Accession: NZ_CP096587
• Organism: Escherichia coli strain EC6622

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: M1B75_RS06420
• Protein ID: WP_000911330.1
• Coordinates: 1315865..1316263 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: M1B75_RS06415
• Protein ID: WP_000450524.1
• Coordinates: 1315638..1315865 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1B75_RS06390 (1311441)	1311441..1311911	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
M1B75_RS06395 (1311911)	1311911..1312483	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
M1B75_RS06400 (1312629)	1312629..1313507	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
M1B75_RS06405 (1313524)	1313524..1314558	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
M1B75_RS06410 (1314771)	1314771..1315484	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
M1B75_RS06415 (1315638)	1315638..1315865	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
M1B75_RS06420 (1315865)	1315865..1316263	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1B75_RS06425 (1316410)	1316410..1317273	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
M1B75_RS06430 (1317288)	1317288..1319303	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
M1B75_RS06435 (1319377)	1319377..1320075	+	699	WP_000679823.1	esterase	-
M1B75_RS06440 (1320185)	1320185..1320385	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T243186 WP_000911330.1 NZ_CP096587:1315865-1316263 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T243186 NZ_OW970413:c4759214-4758930 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT243186 WP_000450524.1 NZ_CP096587:1315638-1315865 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT243186 NZ_OW970413:c4759446-4759204 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33