Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 861170..861824 Replicon chromosome
Accession NZ_CP096587
Organism Escherichia coli strain EC6622

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag M1B75_RS04200 Protein ID WP_000244772.1
Coordinates 861417..861824 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag M1B75_RS04195 Protein ID WP_000354046.1
Coordinates 861170..861436 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1B75_RS04170 (856458) 856458..857201 + 744 WP_000951964.1 SDR family oxidoreductase -
M1B75_RS04175 (857258) 857258..858691 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
M1B75_RS04180 (858736) 858736..859047 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
M1B75_RS04185 (859211) 859211..859870 + 660 WP_000250274.1 hemolysin III family protein -
M1B75_RS04190 (859947) 859947..860927 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
M1B75_RS04195 (861170) 861170..861436 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
M1B75_RS04200 (861417) 861417..861824 + 408 WP_000244772.1 protein YgfX Toxin
M1B75_RS04205 (861864) 861864..862385 - 522 WP_001055874.1 flavodoxin FldB -
M1B75_RS04210 (862497) 862497..863393 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
M1B75_RS04215 (863418) 863418..864128 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
M1B75_RS04220 (864134) 864134..865867 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T243184 WP_000244772.1 NZ_CP096587:861417-861824 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T243184 NZ_OW970413:4040823-4041041 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT243184 WP_000354046.1 NZ_CP096587:861170-861436 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT243184 NZ_OW970413:4040422-4040796 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References