Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 288373..289173 Replicon chromosome
Accession NZ_CP096587
Organism Escherichia coli strain EC6622

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag M1B75_RS01320 Protein ID WP_000342449.1
Coordinates 288646..289173 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag M1B75_RS01315 Protein ID WP_001277108.1
Coordinates 288373..288639 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1B75_RS01295 (284031) 284031..284699 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
M1B75_RS01300 (284692) 284692..285750 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
M1B75_RS01305 (285995) 285995..286849 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
M1B75_RS01310 (287120) 287120..288223 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
M1B75_RS01315 (288373) 288373..288639 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
M1B75_RS01320 (288646) 288646..289173 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
M1B75_RS01325 (289170) 289170..289553 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
M1B75_RS01330 (289977) 289977..291086 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
M1B75_RS01335 (291134) 291134..292060 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
M1B75_RS01340 (292057) 292057..293334 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
M1B75_RS01345 (293331) 293331..294098 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T243182 WP_000342449.1 NZ_CP096587:288646-289173 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T243182 NZ_OW970413:2055596-2055698 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT243182 WP_001277108.1 NZ_CP096587:288373-288639 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT243182 NZ_OW970413:c2055705-2055560 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References