Detailed information of TA system TA243171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3001035..3001637
• Replicon: chromosome
• Accession: NZ_CP096574
• Organism: Geomonas paludis strain RG22

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: M1B72_RS12695
• Protein ID: WP_183347217.1
• Coordinates: 3001341..3001637 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: M1B72_RS12690
• Protein ID: WP_183347216.1
• Coordinates: 3001035..3001337 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1B72_RS12660 (M1B72_12660)	2996671..2997396	+	726	WP_183347205.1	hypothetical protein	-
M1B72_RS12665 (M1B72_12665)	2997592..2997990	+	399	WP_183347207.1	outer membrane protein assembly factor BamE	-
M1B72_RS12670 (M1B72_12670)	2998128..2998703	+	576	WP_183347209.1	GNAT family acetyltransferase	-
M1B72_RS12675 (M1B72_12675)	2998740..2999387	+	648	WP_183347211.1	hypothetical protein	-
M1B72_RS12680 (M1B72_12680)	2999642..3000253	+	612	WP_183347213.1	DUF1294 domain-containing protein	-
M1B72_RS12685 (M1B72_12685)	3000458..3000793	-	336	WP_183347215.1	type II toxin-antitoxin system HicB family antitoxin	-
M1B72_RS12690 (M1B72_12690)	3001035..3001337	-	303	WP_183347216.1	putative addiction module antidote protein	Antitoxin
M1B72_RS12695 (M1B72_12695)	3001341..3001637	-	297	WP_183347217.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M1B72_RS12700 (M1B72_12700)	3001809..3003002	+	1194	WP_183347218.1	hypothetical protein	-
M1B72_RS12705 (M1B72_12705)	3003058..3003420	-	363	WP_183347219.1	hypothetical protein	-
M1B72_RS12710 (M1B72_12710)	3003745..3004329	+	585	WP_183347220.1	glycerol-3-phosphate acyltransferase	-
M1B72_RS12715 (M1B72_12715)	3004347..3006011	+	1665	WP_183347221.1	aminotransferase class V-fold PLP-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2996671..3009610	12939

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-93)

Antitoxin

• Uncharacterized protein HI1420 (8-98)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11200.89 Da
• Isoelectric Point: 9.5883

>T243171 WP_183347217.1 NZ_CP096574:c3001637-3001341 [Geomonas paludis]
MYIVKTLPEFDAWLNGIKDRTTRLRLARRLDKAQRGDLGDVKSVGHGVFEMREFFGSGWRMYYIERGNMLIVMLGGGDKS
TQTADIAIAFERAARLED

Nucleotide

• Length: 297 bp

>T243171 NZ_OW970374:c4733744-4733211 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10655.32 Da
• Isoelectric Point: 7.2777

>AT243171 WP_183347216.1 NZ_CP096574:c3001337-3001035 [Geomonas paludis]
MPKKIVVSDLSEFDLATQLKSEADIAAYITMVIEDGDAAELAHALGVAARARGMAEIAQAAGLTREALYKALRPGAHPRF
DTISRVCSALGVRLVAQPRH

Nucleotide

• Length: 303 bp

>AT243171 NZ_OW970374:c4734021-4733755 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA