Detailed information of TA system TA243165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 121889..122424
• Replicon: plasmid pdjl-6-4
• Accession: NZ_CP096567
• Organism: Rhodococcus qingshengii JCM 15477 strain djl-6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A069J573
• Locus tag: M0639_RS31565
• Protein ID: WP_007733685.1
• Coordinates: 122125..122424 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: M0639_RS31560
• Protein ID: WP_064073730.1
• Coordinates: 121889..122125 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0639_RS31540 (M0639_31540)	118030..118812	+	783	WP_007733691.1	ParA family protein	-
M0639_RS31545 (M0639_31545)	118971..119123	+	153	WP_007733689.1	hypothetical protein	-
M0639_RS31550 (M0639_31550)	119747..120721	+	975	WP_064073729.1	DUF2637 domain-containing protein	-
M0639_RS31555 (M0639_31555)	120831..121457	+	627	WP_248671245.1	TetR/AcrR family transcriptional regulator	-
M0639_RS31560 (M0639_31560)	121889..122125	+	237	WP_064073730.1	YlcI/YnfO family protein	Antitoxin
M0639_RS31565 (M0639_31565)	122125..122424	+	300	WP_007733685.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M0639_RS31570 (M0639_31570)	122453..124303	+	1851	WP_064073731.1	hypothetical protein	-
M0639_RS31575 (M0639_31575)	124303..125100	+	798	WP_064073732.1	hypothetical protein	-
M0639_RS31580 (M0639_31580)	125234..126225	-	992	Protein_116	PDDEXK nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..516925	516925

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10471.13 Da
• Isoelectric Point: 5.0079

>T243165 WP_007733685.1 NZ_CP096567:122125-122424 [Rhodococcus qingshengii JCM 15477]
MRPIHEAKLDTVRPVLVLTREMVRPHLGTVTVAPITGEIRGLSTEVSVSVANGLSADAVVSCDHITTIPVSALGNIIGYL
LPAQEESLSIAVRAAFDLE

Nucleotide

• Length: 300 bp

>T243165 NZ_OW970374:324853-325221 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8768.79 Da
• Isoelectric Point: 6.4825

>AT243165 WP_064073730.1 NZ_CP096567:121889-122125 [Rhodococcus qingshengii JCM 15477]
MSTQIAVRLPDEIVEFLDDEVREHRATSRASLVLRALERERRRQIAARDAEILSAATSEHDLSPLAHHAVNRQPTGST

Nucleotide

• Length: 237 bp

>AT243165 NZ_OW970374:324635-324856 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A069J573