Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2269211..2269725 Replicon chromosome
Accession NZ_CP096540
Organism Staphylococcus aureus strain RIVM_M044329

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag M1F45_RS11460 Protein ID WP_000581792.1
Coordinates 2269211..2269420 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag M1F45_RS11465 Protein ID WP_000138932.1
Coordinates 2269432..2269725 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1F45_RS11440 (M1F45_11425) 2264722..2266077 - 1356 WP_000611457.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
M1F45_RS11445 (M1F45_11430) 2266092..2267162 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
M1F45_RS11450 (M1F45_11435) 2267480..2268682 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
M1F45_RS11455 (M1F45_11440) 2268946..2269083 - 138 WP_000828354.1 Lmo0850 family protein -
M1F45_RS11460 (M1F45_11445) 2269211..2269420 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
M1F45_RS11465 (M1F45_11450) 2269432..2269725 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
M1F45_RS11470 (M1F45_11455) 2269891..2271375 + 1485 WP_000571550.1 cardiolipin synthase -
M1F45_RS11475 (M1F45_11460) 2271403..2272050 + 648 WP_001187614.1 HD domain-containing protein -
M1F45_RS11480 (M1F45_11465) 2272522..2272584 + 63 Protein_2222 hypothetical protein -
M1F45_RS11485 (M1F45_11470) 2272562..2273877 - 1316 Protein_2223 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T243160 WP_000581792.1 NZ_CP096540:c2269420-2269211 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T243160 NZ_OW970365:c4771882-4771598 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT243160 WP_000138932.1 NZ_CP096540:c2269725-2269432 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT243160 NZ_OW970365:c4772114-4771872 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References