Detailed information of TA system TA024316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1983369..1984007
• Replicon: chromosome
• Accession: NC_013361
• Organism: Escherichia coli O26:H11 str. 11368

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ECO26_RS10655
• Protein ID: WP_000813794.1
• Coordinates: 1983369..1983545 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECO26_RS10660
• Protein ID: WP_001270286.1
• Coordinates: 1983591..1984007 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO26_RS10635	1978988..1980202	-	1215	WP_001307190.1	BenE family transporter YdcO	-
ECO26_RS10640	1980255..1980791	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ECO26_RS10645	1980864..1982825	+	1962	WP_001341357.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ECO26_RS10650	1982917..1983147	-	231	WP_000494244.1	YncJ family protein	-
ECO26_RS10655	1983369..1983545	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ECO26_RS10660	1983591..1984007	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ECO26_RS10665	1984086..1985491	+	1406	Protein_2022	PLP-dependent aminotransferase family protein	-
ECO26_RS10670	1985736..1986881	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
ECO26_RS10675	1986899..1987912	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
ECO26_RS10680	1987913..1988854	+	942	WP_001251332.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1977800..1978948	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T24316 WP_000813794.1 NC_013361:1983369-1983545 [Escherichia coli O26:H11 str. 11368]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T24316 NC_013361:1983369-1983545 [Escherichia coli O26:H11 str. 11368]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT24316 WP_001270286.1 NC_013361:1983591-1984007 [Escherichia coli O26:H11 str. 11368]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT24316 NC_013361:1983591-1984007 [Escherichia coli O26:H11 str. 11368]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB