Detailed information of TA system TA243159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2249474..2250003
• Replicon: chromosome
• Accession: NZ_CP096540
• Organism: Staphylococcus aureus strain RIVM_M044329

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: M1F45_RS11370
• Protein ID: WP_000621175.1
• Coordinates: 2249474..2249836 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: M1F45_RS11375
• Protein ID: WP_000948331.1
• Coordinates: 2249833..2250003 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F45_RS11350 (M1F45_11335)	2246452..2247222	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
M1F45_RS11355 (M1F45_11340)	2247197..2247676	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
M1F45_RS11360 (M1F45_11345)	2247678..2248004	-	327	WP_001052491.1	anti-sigma factor antagonist	-
M1F45_RS11365 (M1F45_11350)	2248123..2249124	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
M1F45_RS11370 (M1F45_11355)	2249474..2249836	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M1F45_RS11375 (M1F45_11360)	2249833..2250003	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
M1F45_RS11380 (M1F45_11365)	2250088..2251236	-	1149	WP_001281139.1	alanine racemase	-
M1F45_RS11385 (M1F45_11370)	2251302..2251661	-	360	WP_000581197.1	holo-ACP synthase	-
M1F45_RS11390 (M1F45_11375)	2251665..2252156	-	492	WP_001286980.1	PH domain-containing protein	-
M1F45_RS11395 (M1F45_11380)	2252149..2253726	-	1578	WP_001294650.1	PH domain-containing protein	-
M1F45_RS11400 (M1F45_11385)	2253719..2254198	-	480	WP_001287081.1	hypothetical protein	-
M1F45_RS11405 (M1F45_11390)	2254407..2254967	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T243159 WP_000621175.1 NZ_CP096540:c2249836-2249474 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T243159 NZ_OW970365:c4668330-4667797 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT243159 WP_000948331.1 NZ_CP096540:c2250003-2249833 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT243159 NZ_OW970365:c4668607-4668341 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1