Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 2042398..2043174 Replicon chromosome
Accession NZ_CP096540
Organism Staphylococcus aureus strain RIVM_M044329

Toxin (Protein)


Gene name tsbT Uniprot ID X5E2E6
Locus tag M1F45_RS10175 Protein ID WP_000031108.1
Coordinates 2042398..2042550 (-) Length 51 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID W8U4V4
Locus tag M1F45_RS10180 Protein ID WP_001251224.1
Coordinates 2042575..2043174 (-) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1F45_RS10160 (M1F45_10145) 2038603..2039424 + 822 WP_000669380.1 RluA family pseudouridine synthase -
M1F45_RS10165 (M1F45_10150) 2039879..2041264 - 1386 WP_000116238.1 class II fumarate hydratase -
M1F45_RS10170 (M1F45_10155) 2041460..2041855 - 396 WP_000901018.1 hypothetical protein -
M1F45_RS10175 (M1F45_10160) 2042398..2042550 - 153 WP_000031108.1 SAS053 family protein Toxin
M1F45_RS10180 (M1F45_10165) 2042575..2043174 - 600 WP_001251224.1 glucosamine-6-phosphate isomerase Antitoxin
M1F45_RS10185 (M1F45_10170) 2043333..2043803 - 471 WP_000181394.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
M1F45_RS10190 (M1F45_10175) 2043808..2044935 - 1128 WP_000379980.1 tRNA epoxyqueuosine(34) reductase QueG -
M1F45_RS10195 (M1F45_10180) 2045086..2045808 - 723 WP_031774878.1 amino acid ABC transporter ATP-binding protein -
M1F45_RS10200 (M1F45_10185) 2045801..2047258 - 1458 WP_000649908.1 ABC transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(124-196)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5936.28 Da        Isoelectric Point: 3.8962

>T243156 WP_000031108.1 NZ_CP096540:c2042550-2042398 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Download         Length: 153 bp

>T243156 NZ_OW970365:2054716-2054818 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22343.47 Da        Isoelectric Point: 5.1445

>AT243156 WP_001251224.1 NZ_CP096540:c2043174-2042575 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Download         Length: 600 bp

>AT243156 NZ_OW970365:c2054825-2054680 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X5E2E6

Antitoxin

Source ID Structure
AlphaFold DB W8U4V4

References