Detailed information of TA system TA243151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 959478..959954
• Replicon: chromosome
• Accession: NZ_CP096540
• Organism: Staphylococcus aureus strain RIVM_M044329

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: M1F45_RS04825
• Protein ID: WP_000052076.1
• Coordinates: 959478..959666 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: M1F45_RS04830
• Protein ID: WP_001059690.1
• Coordinates: 959694..959954 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F45_RS04795 (M1F45_04785)	955507..955989	+	483	WP_000824489.1	hypothetical protein	-
M1F45_RS04800 (M1F45_04790)	956330..957016	+	687	WP_001574556.1	thermonuclease family protein	-
M1F45_RS04805 (M1F45_04795)	957373..957573	+	201	WP_001059082.1	cold-shock protein	-
M1F45_RS04810 (M1F45_04800)	958070..958288	-	219	WP_000005881.1	hypothetical protein	-
M1F45_RS04815 (M1F45_04805)	958349..958633	-	285	WP_001004403.1	hypothetical protein	-
M1F45_RS04820 (M1F45_04810)	958721..959290	-	570	WP_001106655.1	lipoprotein N-acylation protein LnsA	-
M1F45_RS04825 (M1F45_04815)	959478..959666	-	189	WP_000052076.1	hypothetical protein	Toxin
M1F45_RS04830 (M1F45_04820)	959694..959954	-	261	WP_001059690.1	hypothetical protein	Antitoxin
M1F45_RS04835 (M1F45_04825)	960296..960499	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
M1F45_RS04840 (M1F45_04830)	960496..960732	-	237	WP_000368687.1	hypothetical protein	-
M1F45_RS04845 (M1F45_04835)	960957..961538	+	582	WP_001140696.1	histidine phosphatase family protein	-
M1F45_RS04850 (M1F45_04840)	961611..962228	-	618	WP_000255562.1	LysE/ArgO family amino acid transporter	-
M1F45_RS04855 (M1F45_04845)	962377..962871	-	495	WP_000569881.1	GNAT family N-acetyltransferase	-
M1F45_RS04860 (M1F45_04850)	963270..963692	-	423	WP_000974452.1	organic hydroperoxide resistance protein	-
M1F45_RS04865 (M1F45_04855)	963840..964556	+	717	WP_000150026.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T243151 WP_000052076.1 NZ_CP096540:c959666-959478 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T243151 NZ_OW970365:332346-332714 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT243151 WP_001059690.1 NZ_CP096540:c959954-959694 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT243151 NZ_OW970365:332128-332349 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56