Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) /HTH_19(antitoxin)
Location 357422..358210 Replicon chromosome
Accession NZ_CP096540
Organism Staphylococcus aureus strain RIVM_M044329

Toxin (Protein)


Gene name - Uniprot ID A0A2I7Y5B3
Locus tag M1F45_RS01585 Protein ID WP_000525004.1
Coordinates 357422..357883 (-) Length 154 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0E7YIA0
Locus tag M1F45_RS01590 Protein ID WP_000333630.1
Coordinates 357896..358210 (-) Length 105 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1F45_RS01560 (M1F45_01555) 352474..354408 + 1935 Protein_309 YSIRK domain-containing triacylglycerol lipase Lip2/Geh -
M1F45_RS01565 (M1F45_01560) 354502..355707 - 1206 WP_031795352.1 tyrosine-type recombinase/integrase -
M1F45_RS01570 (M1F45_01565) 355833..356447 + 615 WP_000191466.1 hypothetical protein -
M1F45_RS01575 (M1F45_01570) 356444..356590 - 147 WP_074370993.1 hypothetical protein -
M1F45_RS01580 (M1F45_01575) 356820..357404 - 585 WP_000825948.1 hypothetical protein -
M1F45_RS01585 (M1F45_01580) 357422..357883 - 462 WP_000525004.1 hypothetical protein Toxin
M1F45_RS01590 (M1F45_01585) 357896..358210 - 315 WP_000333630.1 helix-turn-helix transcriptional regulator Antitoxin
M1F45_RS01595 (M1F45_01590) 358362..358598 + 237 WP_001121027.1 helix-turn-helix transcriptional regulator -
M1F45_RS01600 (M1F45_01595) 358612..359388 + 777 WP_001148544.1 Rha family transcriptional regulator -
M1F45_RS01605 (M1F45_01600) 359417..359560 + 144 WP_031797335.1 hypothetical protein -
M1F45_RS01610 (M1F45_01605) 359550..359759 - 210 WP_000642492.1 hypothetical protein -
M1F45_RS01615 (M1F45_01610) 359815..360060 + 246 WP_001025401.1 DUF2829 domain-containing protein -
M1F45_RS01620 (M1F45_01615) 360029..360394 - 366 WP_001128433.1 hypothetical protein -
M1F45_RS01625 (M1F45_01620) 360449..360664 + 216 WP_001097552.1 hypothetical protein -
M1F45_RS01630 (M1F45_01625) 360689..360952 + 264 WP_001124160.1 helix-turn-helix domain-containing protein -
M1F45_RS01635 (M1F45_01630) 360965..361135 + 171 WP_031920869.1 DUF1270 domain-containing protein -
M1F45_RS01640 (M1F45_01635) 361116..361454 + 339 WP_224727996.1 XRE family transcriptional regulator -
M1F45_RS01645 (M1F45_01640) 361519..361821 + 303 WP_031838380.1 DUF2482 family protein -
M1F45_RS01650 (M1F45_01645) 361826..362086 + 261 WP_000291481.1 DUF1108 family protein -
M1F45_RS01655 (M1F45_01650) 362099..362635 + 537 WP_000999048.1 host-nuclease inhibitor Gam family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 354502..401166 46664


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

(4-62)


Sequences


Toxin        


Download         Length: 154 a.a.        Molecular weight: 18074.46 Da        Isoelectric Point: 4.6915

>T243146 WP_000525004.1 NZ_CP096540:c357883-357422 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Download         Length: 462 bp

>T243146 NZ_OW970357:c4768823-4768539 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12063.56 Da        Isoelectric Point: 6.4736

>AT243146 WP_000333630.1 NZ_CP096540:c358210-357896 [Staphylococcus aureus]
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYADYIRSKRK

Download         Length: 315 bp

>AT243146 NZ_OW970357:c4769055-4768813 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2I7Y5B3


Antitoxin

Source ID Structure
AlphaFold DB A0A0E7YIA0

References