Detailed information of TA system TA243141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2122773..2123302
• Replicon: chromosome
• Accession: NZ_CP096539
• Organism: Staphylococcus aureus strain RIVM_M047065

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: M1F46_RS10445
• Protein ID: WP_000621175.1
• Coordinates: 2122773..2123135 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: M1F46_RS10450
• Protein ID: WP_000948331.1
• Coordinates: 2123132..2123302 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F46_RS10425 (M1F46_10420)	2119751..2120521	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
M1F46_RS10430 (M1F46_10425)	2120496..2120975	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
M1F46_RS10435 (M1F46_10430)	2120977..2121303	-	327	WP_001052491.1	anti-sigma factor antagonist	-
M1F46_RS10440 (M1F46_10435)	2121422..2122423	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
M1F46_RS10445 (M1F46_10440)	2122773..2123135	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M1F46_RS10450 (M1F46_10445)	2123132..2123302	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
M1F46_RS10455 (M1F46_10450)	2123387..2124535	-	1149	WP_001281139.1	alanine racemase	-
M1F46_RS10460 (M1F46_10455)	2124601..2124960	-	360	WP_000581197.1	holo-ACP synthase	-
M1F46_RS10465 (M1F46_10460)	2124964..2125455	-	492	WP_001286980.1	PH domain-containing protein	-
M1F46_RS10470 (M1F46_10465)	2125448..2127025	-	1578	WP_001294650.1	PH domain-containing protein	-
M1F46_RS10475 (M1F46_10470)	2127018..2127497	-	480	WP_001287081.1	hypothetical protein	-
M1F46_RS10480 (M1F46_10475)	2127706..2128266	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T243141 WP_000621175.1 NZ_CP096539:c2123135-2122773 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T243141 NZ_OW970357:1514066-1514548 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT243141 WP_000948331.1 NZ_CP096539:c2123302-2123132 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT243141 NZ_OW970357:1513812-1514078 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1