Detailed information of TA system TA243140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1961126..1961902
• Replicon: chromosome
• Accession: NZ_CP096539
• Organism: Staphylococcus aureus strain RIVM_M047065

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: M1F46_RS09525
• Protein ID: WP_000031108.1
• Coordinates: 1961126..1961278 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: M1F46_RS09530
• Protein ID: WP_001251224.1
• Coordinates: 1961303..1961902 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F46_RS09510 (M1F46_09505)	1957331..1958152	+	822	WP_000669380.1	RluA family pseudouridine synthase	-
M1F46_RS09515 (M1F46_09510)	1958607..1959992	-	1386	WP_000116238.1	class II fumarate hydratase	-
M1F46_RS09520 (M1F46_09515)	1960188..1960583	-	396	WP_000901018.1	hypothetical protein	-
M1F46_RS09525 (M1F46_09520)	1961126..1961278	-	153	WP_000031108.1	SAS053 family protein	Toxin
M1F46_RS09530 (M1F46_09525)	1961303..1961902	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
M1F46_RS09535 (M1F46_09530)	1962061..1962531	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
M1F46_RS09540 (M1F46_09535)	1962536..1963663	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
M1F46_RS09545 (M1F46_09540)	1963814..1964536	-	723	WP_031774878.1	amino acid ABC transporter ATP-binding protein	-
M1F46_RS09550 (M1F46_09545)	1964529..1965986	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T243140 WP_000031108.1 NZ_CP096539:c1961278-1961126 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T243140 NZ_OW970357:1497348-1497764 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT243140 WP_001251224.1 NZ_CP096539:c1961902-1961303 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT243140 NZ_OW970357:1497121-1497351 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4