Detailed information of TA system TA243135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 875003..875479
• Replicon: chromosome
• Accession: NZ_CP096539
• Organism: Staphylococcus aureus strain RIVM_M047065

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: M1F46_RS04150
• Protein ID: WP_000052076.1
• Coordinates: 875003..875191 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: M1F46_RS04155
• Protein ID: WP_001059690.1
• Coordinates: 875219..875479 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F46_RS04120 (M1F46_04115)	871032..871514	+	483	WP_000824489.1	hypothetical protein	-
M1F46_RS04125 (M1F46_04120)	871855..872541	+	687	WP_001574556.1	thermonuclease family protein	-
M1F46_RS04130 (M1F46_04125)	872898..873098	+	201	WP_001059082.1	cold-shock protein	-
M1F46_RS04135 (M1F46_04130)	873595..873813	-	219	WP_000005881.1	hypothetical protein	-
M1F46_RS04140 (M1F46_04135)	873874..874158	-	285	WP_001004403.1	hypothetical protein	-
M1F46_RS04145 (M1F46_04140)	874246..874815	-	570	WP_001106655.1	lipoprotein N-acylation protein LnsA	-
M1F46_RS04150 (M1F46_04145)	875003..875191	-	189	WP_000052076.1	hypothetical protein	Toxin
M1F46_RS04155 (M1F46_04150)	875219..875479	-	261	WP_001059690.1	hypothetical protein	Antitoxin
M1F46_RS04160 (M1F46_04155)	875821..876024	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
M1F46_RS04165 (M1F46_04160)	876021..876257	-	237	WP_000368687.1	hypothetical protein	-
M1F46_RS04170 (M1F46_04165)	876482..877063	+	582	WP_001140696.1	histidine phosphatase family protein	-
M1F46_RS04175 (M1F46_04170)	877136..877753	-	618	WP_000255562.1	LysE/ArgO family amino acid transporter	-
M1F46_RS04180 (M1F46_04175)	877902..878396	-	495	WP_000569881.1	GNAT family N-acetyltransferase	-
M1F46_RS04185 (M1F46_04180)	878795..879217	-	423	WP_000974452.1	organic hydroperoxide resistance protein	-
M1F46_RS04190 (M1F46_04185)	879365..880081	+	717	WP_000150026.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (2-78)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T243135 WP_000052076.1 NZ_CP096539:c875191-875003 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T243135 NZ_OW970318:c31615-31328 [Pantoea agglomerans]
ATGCCCTGGCAGCTTGAGTGTGATCAGAGGGCACTGAAAGAGTGGCGTAAGCTGGGCCCGACAGTACGTGAACAGCTTAA
AAAGAAGCTGGCTGAGTGTCTTGAGTCACCCAGAATTGAGGCGAACAAACTCAGCAGGATGCCTGACTGCTATAAGATCA
AGCTAAAGTCATCCGGTTATCGACTTGCATACCAGGTTATTGATGACCGGGTGGTCGTTTTTGTGGTGGCTGTCGGGAAA
AGAGAGCGCTCAGAGGTCTGTTCTGCCGCTTCCAGGCGTCTCTCTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT243135 WP_001059690.1 NZ_CP096539:c875479-875219 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT243135 NZ_OW970318:c31854-31615 [Pantoea agglomerans]
GTGGGTTCAATTAGCATCCGAATTGATGGTGATCTTAAGAGACGATCCTTTGCTGCGCTCAAAAAAATGGGTGTGACGCC
CTCTGACGCGCTGCGCCTTATGCTTGCATATATTGCAGATAATGAACGTCTTTCCTTTAAGCAGACATCATTCAGTGACG
AAGATGCGGCGTTAGTAGACATAGTGAAAGAACGCCTGCGAAATCCTGAGCCTGTCAGGATCACGCTGGACAAGCTTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56