Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2180836..2181350 Replicon chromosome
Accession NZ_CP096535
Organism Staphylococcus aureus strain RIVM_M047916

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag M1F47_RS10845 Protein ID WP_000581792.1
Coordinates 2180836..2181045 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag M1F47_RS10850 Protein ID WP_000138932.1
Coordinates 2181057..2181350 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1F47_RS10825 (M1F47_10810) 2176347..2177702 - 1356 WP_000611457.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
M1F47_RS10830 (M1F47_10815) 2177717..2178787 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
M1F47_RS10835 (M1F47_10820) 2179105..2180307 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
M1F47_RS10840 (M1F47_10825) 2180571..2180708 - 138 WP_000828354.1 Lmo0850 family protein -
M1F47_RS10845 (M1F47_10830) 2180836..2181045 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
M1F47_RS10850 (M1F47_10835) 2181057..2181350 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
M1F47_RS10855 (M1F47_10840) 2181516..2183000 + 1485 WP_000571550.1 cardiolipin synthase -
M1F47_RS10860 (M1F47_10845) 2183028..2183675 + 648 WP_001187614.1 HD domain-containing protein -
M1F47_RS10865 (M1F47_10850) 2184232..2184294 + 63 Protein_2099 hypothetical protein -
M1F47_RS10870 (M1F47_10855) 2184272..2185587 - 1316 Protein_2100 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T243131 WP_000581792.1 NZ_CP096535:c2181045-2180836 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T243131 NZ_OW970315:3149110-3149325 [Pantoea agglomerans]
ATGAACAAAACCCTGACTAAAACCGATTATTTGATGCGACTGCGGCGCTGCCGCTCTCTTGATACCCTGGAAAGGGTGAT
TGAAAAAAATAAGTATGAATTACCGGAAGATGAATTAGCGGTATTTTACTCAGCAGCTGACCATCGCCTGGCTGAACTGA
CGATGAACAAGCTCTACGATAAAGTCCCGGGTTCAGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT243131 WP_000138932.1 NZ_CP096535:c2181350-2181057 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT243131 NZ_OW970315:3148708-3149085 [Pantoea agglomerans]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACCTTTGTGAGAGTCTGTTTGACGACAGCATGGC
AACATTAACCGACAGCCATCATGGCTGGGTTAATGATCCCACTTCTGAAAGCAATCTACAGCTTAACGATTTGATTGAGC
ATATCGCCTCTTTCACTATGAATTACAAAATTAAGCACGTTGAAGATGAAGCGCTGATTTCGCAAATCGATGAATATCTT
GATGATACTTTTATGCTATTCAGCAATTATGGTGTCAATAGTCCGGATCTTCAGCGGTGGCAACGTTCAGCAAAACGCTT
ATTTAATCTGTTCACTGAAGAGTGCGCTTTTCTCCAGCAACCGAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References