Detailed information of TA system TA243130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2161099..2161628
• Replicon: chromosome
• Accession: NZ_CP096535
• Organism: Staphylococcus aureus strain RIVM_M047916

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: M1F47_RS10755
• Protein ID: WP_000621175.1
• Coordinates: 2161099..2161461 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: M1F47_RS10760
• Protein ID: WP_000948331.1
• Coordinates: 2161458..2161628 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F47_RS10735 (M1F47_10720)	2158077..2158847	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
M1F47_RS10740 (M1F47_10725)	2158822..2159301	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
M1F47_RS10745 (M1F47_10730)	2159303..2159629	-	327	WP_001052491.1	anti-sigma factor antagonist	-
M1F47_RS10750 (M1F47_10735)	2159748..2160749	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
M1F47_RS10755 (M1F47_10740)	2161099..2161461	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M1F47_RS10760 (M1F47_10745)	2161458..2161628	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
M1F47_RS10765 (M1F47_10750)	2161713..2162861	-	1149	WP_001281139.1	alanine racemase	-
M1F47_RS10770 (M1F47_10755)	2162927..2163286	-	360	WP_000581197.1	holo-ACP synthase	-
M1F47_RS10775 (M1F47_10760)	2163290..2163781	-	492	WP_001286980.1	PH domain-containing protein	-
M1F47_RS10780 (M1F47_10765)	2163774..2165351	-	1578	WP_001294650.1	PH domain-containing protein	-
M1F47_RS10785 (M1F47_10770)	2165344..2165823	-	480	WP_001287081.1	hypothetical protein	-
M1F47_RS10790 (M1F47_10775)	2166032..2166592	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T243130 WP_000621175.1 NZ_CP096535:c2161461-2161099 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T243130 NZ_OW970315:2116395-2117708 [Pantoea agglomerans]
ATGCAAAGGCTGATTGCGTGGATGAACGGCGAGCGGGTAGGCGTACTCACCCGCCAGCGAAACGGCGCTCATGAGTTTCA
GTATGATACCGCCTGGCTGAGTAATCCGCTCTCCAGGCCGCTGTCACTTTCGATGCCGTTGCAGGTTAAAGCGATCACCT
CACACGCCGTGATTAACTTCTTCGATAACCTGCTGCCGGATAGCCCATTGATTCGCGACAGAATCGTGGCACGTTACCAG
ATTAAATCCCGTCAGCCGTTTGATCTGTTGCAGGAAGTGGGACGTGACAGCGTCGGGGCAACCATGCTGCTGCCGCCCGA
TATGCCACTGCCCGCACTGCAGGCCCGCTATGAAGTGCTGGATGAAACCCGGCTGGAAAGCGTGCTGTCGGCCTATCAGT
CAGATATCCCGCTGGGGATGCTGCGTGAAGAGACCGATTTCCGCATCTCGGTCGCGGGGGCGCAGGAGAAAACTGCACTG
CTGCTGACCGATGAAGGCTGGAGCATTCCTGCCGGTGCCACGCCCACCTCGCACATCATTAAGCTGCCGATCGGAGAGAT
TAAACAGCCGGCCGCGACGCTGGATATGCGCGAAAGTGTGGAGAATGAGTATCTCTGTCTGGCGCTGGCCCGTGCACTCG
GTATCGATGTGCCGCAGGCAACGATTATCCGTGCCGGACAGATGCGCGCCCTGGCGGTAGAACGTTTCGACCGCCGCTGG
TCACGCGATAAAACCCGGTTGATCCGCCTGCCGCAGGAGGATTTCTGTCAGGCGCTGGGACTGCCATCTTCAACCAAATA
TGAGTCGGATGGCGGGCCGGGCATTGCGGAGATCATGCAGATACTGATGGGATCGAGCAACGCCATCGAAGATCGCTATC
GCTTTATGATGTTCCAGGTGTTTCAGTGGCTGATTGGCGCAACCGACGGTCACGCCAAGAACTTCTCACTGTTTATTGAG
CGCGGCGGCAGTTATCGACTGACTCCCTTTTATGATGTGATCTCCGCTTACCCGGTACTGGGCGGCACCGGTCTGAACAA
GCGAGCGCTGAAACTGGCGATGGGTCTGCGTGCCACTAAGGGGAAGAAGACAGAGATCGACAAAATCTTCCCGCGCCATT
TTATCGCCACGGCAAAAAGCGTCAGTTTTGATCGTGACAGTTTGCAGCAGATCTTCGACTTCTTTATTAAGGCCTTTCCC
CAGGCGGTGGTCTCGGTGCGCGATGCTTTGCCGCCAGGTTTCCCGCAGCACATCGCCGACGCCATTTTCAGTCATTCACT
GATTATGCTGGAGCGGCTGCGGCACAAAGAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT243130 WP_000948331.1 NZ_CP096535:c2161628-2161458 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT243130 NZ_OW970315:2116147-2116395 [Pantoea agglomerans]
ATGATATACAGCCCGCTACAGCTGGCAAACCGGCTAAAGCTCATTCGTCAGCGCAATGGCTGGACCCAGAGTGAGCTGGC
GAAAAAGGTCGGCCTGAAACAGGCTACCATCTCTCACTTTGAAAATGCCCCTGACAGCACCTCGCTGGCAACGCTGTTTA
AGCTGTTACAGTCGCTGGAGCTGAGTCTGGAAGTTCAGGAGAAAGAGGAAGCCAGTGCGCCTCAGCTGCATGATGAGGAT
GAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1