Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 1999452..2000228 Replicon chromosome
Accession NZ_CP096535
Organism Staphylococcus aureus strain RIVM_M047916

Toxin (Protein)


Gene name tsbT Uniprot ID X5E2E6
Locus tag M1F47_RS09835 Protein ID WP_000031108.1
Coordinates 1999452..1999604 (-) Length 51 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID W8U4V4
Locus tag M1F47_RS09840 Protein ID WP_001251224.1
Coordinates 1999629..2000228 (-) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1F47_RS09820 (M1F47_09805) 1995657..1996478 + 822 WP_000669380.1 RluA family pseudouridine synthase -
M1F47_RS09825 (M1F47_09810) 1996933..1998318 - 1386 WP_000116238.1 class II fumarate hydratase -
M1F47_RS09830 (M1F47_09815) 1998514..1998909 - 396 WP_000901018.1 hypothetical protein -
M1F47_RS09835 (M1F47_09820) 1999452..1999604 - 153 WP_000031108.1 SAS053 family protein Toxin
M1F47_RS09840 (M1F47_09825) 1999629..2000228 - 600 WP_001251224.1 glucosamine-6-phosphate isomerase Antitoxin
M1F47_RS09845 (M1F47_09830) 2000387..2000857 - 471 WP_000181394.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
M1F47_RS09850 (M1F47_09835) 2000862..2001989 - 1128 WP_000379980.1 tRNA epoxyqueuosine(34) reductase QueG -
M1F47_RS09855 (M1F47_09840) 2002140..2002862 - 723 WP_031774878.1 amino acid ABC transporter ATP-binding protein -
M1F47_RS09860 (M1F47_09845) 2002855..2004312 - 1458 WP_000649908.1 ABC transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(124-196)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5936.28 Da        Isoelectric Point: 3.8962

>T243129 WP_000031108.1 NZ_CP096535:c1999604-1999452 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Download         Length: 153 bp

>T243129 NZ_OW969913:82938-83225 [Klebsiella variicola]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22343.47 Da        Isoelectric Point: 5.1445

>AT243129 WP_001251224.1 NZ_CP096535:c2000228-1999629 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Download         Length: 600 bp

>AT243129 NZ_OW969913:82699-82938 [Klebsiella variicola]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X5E2E6

Antitoxin

Source ID Structure
AlphaFold DB W8U4V4

References