Detailed information of TA system TA243125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 872742..873218
• Replicon: chromosome
• Accession: NZ_CP096535
• Organism: Staphylococcus aureus strain RIVM_M047916

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: M1F47_RS04145
• Protein ID: WP_000052076.1
• Coordinates: 872742..872930 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: M1F47_RS04150
• Protein ID: WP_001059690.1
• Coordinates: 872958..873218 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F47_RS04115 (M1F47_04110)	868771..869253	+	483	WP_000824489.1	hypothetical protein	-
M1F47_RS04120 (M1F47_04115)	869594..870280	+	687	WP_001574556.1	thermonuclease family protein	-
M1F47_RS04125 (M1F47_04120)	870637..870837	+	201	WP_001059082.1	cold-shock protein	-
M1F47_RS04130 (M1F47_04125)	871334..871552	-	219	WP_000005881.1	hypothetical protein	-
M1F47_RS04135 (M1F47_04130)	871613..871897	-	285	WP_001004403.1	hypothetical protein	-
M1F47_RS04140 (M1F47_04135)	871985..872554	-	570	WP_001106655.1	lipoprotein N-acylation protein LnsA	-
M1F47_RS04145 (M1F47_04140)	872742..872930	-	189	WP_000052076.1	hypothetical protein	Toxin
M1F47_RS04150 (M1F47_04145)	872958..873218	-	261	WP_001059690.1	hypothetical protein	Antitoxin
M1F47_RS04155 (M1F47_04150)	873560..873763	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
M1F47_RS04160 (M1F47_04155)	873760..873996	-	237	WP_000368687.1	hypothetical protein	-
M1F47_RS04165 (M1F47_04160)	874221..874802	+	582	WP_001140696.1	histidine phosphatase family protein	-
M1F47_RS04170 (M1F47_04165)	874875..875492	-	618	WP_000255562.1	LysE/ArgO family amino acid transporter	-
M1F47_RS04175 (M1F47_04170)	875641..876135	-	495	WP_000569881.1	GNAT family N-acetyltransferase	-
M1F47_RS04180 (M1F47_04175)	876534..876956	-	423	WP_000974452.1	organic hydroperoxide resistance protein	-
M1F47_RS04185 (M1F47_04180)	877104..877820	+	717	WP_000150026.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T243125 WP_000052076.1 NZ_CP096535:c872930-872742 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T243125 NZ_OW969912:c4880219-4879935 [Klebsiella variicola]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT243125 WP_001059690.1 NZ_CP096535:c873218-872958 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT243125 NZ_OW969912:c4880451-4880209 [Klebsiella variicola]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56