Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2229442..2229956 Replicon chromosome
Accession NZ_CP096532
Organism Staphylococcus aureus strain RIVM_M083782

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag M1F48_RS11160 Protein ID WP_000581792.1
Coordinates 2229442..2229651 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag M1F48_RS11165 Protein ID WP_000138932.1
Coordinates 2229663..2229956 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1F48_RS11140 (M1F48_11115) 2224953..2226308 - 1356 WP_000611457.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
M1F48_RS11145 (M1F48_11120) 2226323..2227393 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
M1F48_RS11150 (M1F48_11125) 2227711..2228913 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
M1F48_RS11155 (M1F48_11130) 2229177..2229314 - 138 WP_000828354.1 Lmo0850 family protein -
M1F48_RS11160 (M1F48_11135) 2229442..2229651 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
M1F48_RS11165 (M1F48_11140) 2229663..2229956 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
M1F48_RS11170 (M1F48_11145) 2230122..2231606 + 1485 WP_000571550.1 cardiolipin synthase -
M1F48_RS11175 (M1F48_11150) 2231634..2232281 + 648 WP_001187614.1 HD domain-containing protein -
M1F48_RS11180 (M1F48_11155) 2232838..2232900 + 63 Protein_2162 hypothetical protein -
M1F48_RS11185 (M1F48_11160) 2232878..2234193 - 1316 Protein_2163 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T243121 WP_000581792.1 NZ_CP096532:c2229651-2229442 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T243121 NZ_OW969912:1948357-1948459 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT243121 WP_000138932.1 NZ_CP096532:c2229956-2229663 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT243121 NZ_OW969912:c1948465-1948321 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References