Detailed information of TA system TA243120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2209705..2210234
• Replicon: chromosome
• Accession: NZ_CP096532
• Organism: Staphylococcus aureus strain RIVM_M083782

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: M1F48_RS11070
• Protein ID: WP_000621175.1
• Coordinates: 2209705..2210067 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: M1F48_RS11075
• Protein ID: WP_000948331.1
• Coordinates: 2210064..2210234 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F48_RS11050 (M1F48_11025)	2206683..2207453	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
M1F48_RS11055 (M1F48_11030)	2207428..2207907	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
M1F48_RS11060 (M1F48_11035)	2207909..2208235	-	327	WP_001052491.1	anti-sigma factor antagonist	-
M1F48_RS11065 (M1F48_11040)	2208354..2209355	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
M1F48_RS11070 (M1F48_11045)	2209705..2210067	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M1F48_RS11075 (M1F48_11050)	2210064..2210234	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
M1F48_RS11080 (M1F48_11055)	2210319..2211467	-	1149	WP_001281139.1	alanine racemase	-
M1F48_RS11085 (M1F48_11060)	2211533..2211892	-	360	WP_000581197.1	holo-ACP synthase	-
M1F48_RS11090 (M1F48_11065)	2211896..2212387	-	492	WP_001286980.1	PH domain-containing protein	-
M1F48_RS11095 (M1F48_11070)	2212380..2213957	-	1578	WP_001294650.1	PH domain-containing protein	-
M1F48_RS11100 (M1F48_11075)	2213950..2214429	-	480	WP_001287081.1	hypothetical protein	-
M1F48_RS11105 (M1F48_11080)	2214638..2215198	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T243120 WP_000621175.1 NZ_CP096532:c2210067-2209705 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T243120 NZ_OW969912:1770375-1770707 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT243120 WP_000948331.1 NZ_CP096532:c2210234-2210064 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT243120 NZ_OW969912:1770117-1770374 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1