Detailed information of TA system TA243119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 2046984..2047760
• Replicon: chromosome
• Accession: NZ_CP096532
• Organism: Staphylococcus aureus strain RIVM_M083782

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: M1F48_RS10145
• Protein ID: WP_000031108.1
• Coordinates: 2046984..2047136 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: M1F48_RS10150
• Protein ID: WP_001251224.1
• Coordinates: 2047161..2047760 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F48_RS10130 (M1F48_10105)	2043189..2044010	+	822	WP_000669380.1	RluA family pseudouridine synthase	-
M1F48_RS10135 (M1F48_10110)	2044465..2045850	-	1386	WP_000116238.1	class II fumarate hydratase	-
M1F48_RS10140 (M1F48_10115)	2046046..2046441	-	396	WP_000901018.1	hypothetical protein	-
M1F48_RS10145 (M1F48_10120)	2046984..2047136	-	153	WP_000031108.1	SAS053 family protein	Toxin
M1F48_RS10150 (M1F48_10125)	2047161..2047760	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
M1F48_RS10155 (M1F48_10130)	2047919..2048389	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
M1F48_RS10160 (M1F48_10135)	2048394..2049521	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
M1F48_RS10165 (M1F48_10140)	2049672..2050394	-	723	WP_031774878.1	amino acid ABC transporter ATP-binding protein	-
M1F48_RS10170 (M1F48_10145)	2050387..2051844	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T243119 WP_000031108.1 NZ_CP096532:c2047136-2046984 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T243119 NZ_OW969912:787714-788052 [Klebsiella variicola]
ATGAAAACTCTTCCTGCAAATCAGCGGGTGGCGAAGCCATGCCCGCCGCCTGTGCTCGTCTGGCAAACGCTACTTACACG
CCTGCTGGAGCAACACTATGGCCTGACGCTGAACGATACGCCGTTCAGTGATGAGAACGTTATTCAGGAGCATATCAACG
CTGGTATCACGCTGGCGGATGCCGTTAACTTTCTGGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGGGGGTTTAAC
TGTCAGGAGCAGTCACCTTACCTTCGGGCGGTAGATATTTTACGGGCGAGGCAGGCAACTGGCCTGTTGCGGCAAAGCCA
GCTTCCTTCGATACGCTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT243119 WP_001251224.1 NZ_CP096532:c2047760-2047161 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT243119 NZ_OW969912:787363-787689 [Klebsiella variicola]
ATGGAAAAACACGTTAACCCTGAATGGGGGCTTCGATGCGTGGTAATGCCTCGCTTTGGTGCACGTCTGGTACAGGAAGG
CAACCGACTACATTATTTAGCTGACCGCGCTGGGATAACTGGGACTTTCAATGAAGAACAGTGTCACCAACTGAATAGCG
CATTTCCGCACTTTATAGAGCAGATGGAACTGATGCTGCTCTCTGGTGAGCTTAACCCCCGCCATGCACATCGCGTAACG
CTGTACCACAACGAACTGACCTGCGAAGCGGATACGCTCGGTAGCTGTGGCTACGTTTATATCGTTATCTATCCCACTCA
ACGTTAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4