Detailed information of TA system TA243115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 965021..965497
• Replicon: chromosome
• Accession: NZ_CP096532
• Organism: Staphylococcus aureus strain RIVM_M083782

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: M1F48_RS04835
• Protein ID: WP_000052076.1
• Coordinates: 965021..965209 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: M1F48_RS04840
• Protein ID: WP_001059690.1
• Coordinates: 965237..965497 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F48_RS04805 (M1F48_04790)	961050..961532	+	483	WP_000824489.1	hypothetical protein	-
M1F48_RS04810 (M1F48_04795)	961873..962559	+	687	WP_001574556.1	thermonuclease family protein	-
M1F48_RS04815 (M1F48_04800)	962916..963116	+	201	WP_001059082.1	cold-shock protein	-
M1F48_RS04820 (M1F48_04805)	963613..963831	-	219	WP_000005881.1	hypothetical protein	-
M1F48_RS04825 (M1F48_04810)	963892..964176	-	285	WP_001004403.1	hypothetical protein	-
M1F48_RS04830 (M1F48_04815)	964264..964833	-	570	WP_001106655.1	lipoprotein N-acylation protein LnsA	-
M1F48_RS04835 (M1F48_04820)	965021..965209	-	189	WP_000052076.1	hypothetical protein	Toxin
M1F48_RS04840 (M1F48_04825)	965237..965497	-	261	WP_001059690.1	hypothetical protein	Antitoxin
M1F48_RS04845 (M1F48_04830)	965839..966042	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
M1F48_RS04850 (M1F48_04835)	966039..966275	-	237	WP_000368687.1	hypothetical protein	-
M1F48_RS04855 (M1F48_04840)	966500..967081	+	582	WP_001140696.1	histidine phosphatase family protein	-
M1F48_RS04860 (M1F48_04845)	967154..967771	-	618	WP_000255562.1	LysE/ArgO family amino acid transporter	-
M1F48_RS04865 (M1F48_04850)	967920..968414	-	495	WP_000569881.1	GNAT family N-acetyltransferase	-
M1F48_RS04870 (M1F48_04855)	968813..969235	-	423	WP_000974452.1	organic hydroperoxide resistance protein	-
M1F48_RS04875 (M1F48_04860)	969383..970099	+	717	WP_000150026.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	vWbp	907158..970099	62941

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T243115 WP_000052076.1 NZ_CP096532:c965209-965021 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T243115 NZ_OW969912:274137-274412 [Klebsiella variicola]
ATGGAGCAGCAGGTTTTATCTTTACGTAATAAACAGCGCCACACGCTGGAGCAACTGTTTAAAACCCCTGTCCCACAGGG
CATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGCGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCATCCTTCGCCGGATACCGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAGACCATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT243115 WP_001059690.1 NZ_CP096532:c965497-965237 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT243115 NZ_OW969912:274409-274768 [Klebsiella variicola]
ATGATCAAACCTAAAACGCCAAACTCAATGGAGATTGCCGGCCAGCCTGCCGTTATCAATTACGTGCCTGAGCTCAATGT
ATTCCGCGGTAAGTTCCTGGGATTATCAGGATATTGCGATTTTGTTTCCGATAGTATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAGTATCTCGCAGATTGTCATGAAGCGGGGATTGAGCCTTATGCTCACCCGGAAAAGATGAAAACC
TTCACGCTGCGCTATCCTGAGTCATTTGGTGAGCGTCTTAGCTCCGCCGCTGCAGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCATAA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56