Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | /HTH_19(antitoxin) |
| Location | 365095..365883 | Replicon | chromosome |
| Accession | NZ_CP096532 | ||
| Organism | Staphylococcus aureus strain RIVM_M083782 | ||
Toxin (Protein)
| Gene name | - | Uniprot ID | A0A2I7Y5B3 |
| Locus tag | M1F48_RS01625 | Protein ID | WP_000525004.1 |
| Coordinates | 365095..365556 (-) | Length | 154 a.a. |
Antitoxin (Protein)
| Gene name | - | Uniprot ID | A0A0E7YIA0 |
| Locus tag | M1F48_RS01630 | Protein ID | WP_000333630.1 |
| Coordinates | 365569..365883 (-) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| M1F48_RS01600 (M1F48_01595) | 360135..362069 | + | 1935 | Protein_317 | YSIRK domain-containing triacylglycerol lipase Lip2/Geh | - |
| M1F48_RS01605 (M1F48_01600) | 362163..363368 | - | 1206 | WP_031920108.1 | tyrosine-type recombinase/integrase | - |
| M1F48_RS01610 (M1F48_01605) | 363494..364108 | + | 615 | WP_000191459.1 | hypothetical protein | - |
| M1F48_RS01615 (M1F48_01610) | 364105..364251 | - | 147 | WP_078067907.1 | hypothetical protein | - |
| M1F48_RS01620 (M1F48_01615) | 364481..365077 | - | 597 | WP_031796639.1 | hypothetical protein | - |
| M1F48_RS01625 (M1F48_01620) | 365095..365556 | - | 462 | WP_000525004.1 | hypothetical protein | Toxin |
| M1F48_RS01630 (M1F48_01625) | 365569..365883 | - | 315 | WP_000333630.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| M1F48_RS01635 (M1F48_01630) | 366035..366271 | + | 237 | WP_001121027.1 | helix-turn-helix transcriptional regulator | - |
| M1F48_RS01640 (M1F48_01635) | 366285..367061 | + | 777 | WP_001148544.1 | Rha family transcriptional regulator | - |
| M1F48_RS01645 (M1F48_01640) | 367090..367233 | + | 144 | WP_000939498.1 | hypothetical protein | - |
| M1F48_RS01650 (M1F48_01645) | 367293..367478 | + | 186 | Protein_327 | hypothetical protein | - |
| M1F48_RS01655 (M1F48_01650) | 367493..367642 | + | 150 | WP_000771849.1 | hypothetical protein | - |
| M1F48_RS01660 (M1F48_01655) | 367683..367895 | - | 213 | WP_000362645.1 | hypothetical protein | - |
| M1F48_RS01665 (M1F48_01660) | 367964..368161 | + | 198 | WP_031838117.1 | hypothetical protein | - |
| M1F48_RS01670 (M1F48_01665) | 368148..368529 | - | 382 | Protein_331 | DUF2513 domain-containing protein | - |
| M1F48_RS01675 (M1F48_01670) | 368596..368841 | + | 246 | WP_001025401.1 | DUF2829 domain-containing protein | - |
| M1F48_RS01680 (M1F48_01675) | 368810..369175 | - | 366 | WP_001128433.1 | hypothetical protein | - |
| M1F48_RS01685 (M1F48_01680) | 369230..369445 | + | 216 | WP_001097552.1 | hypothetical protein | - |
| M1F48_RS01690 (M1F48_01685) | 369470..369733 | + | 264 | WP_016187436.1 | helix-turn-helix domain-containing protein | - |
| M1F48_RS01695 (M1F48_01690) | 369746..369907 | + | 162 | WP_001285948.1 | DUF1270 domain-containing protein | - |
| M1F48_RS01700 (M1F48_01695) | 369986..370309 | + | 324 | WP_000174994.1 | hypothetical protein | - |
| M1F48_RS01705 (M1F48_01700) | 370324..370686 | + | 363 | WP_000985976.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 362163..409043 | 46880 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 154 a.a. Molecular weight: 18074.46 Da Isoelectric Point: 4.6915
>T243111 WP_000525004.1 NZ_CP096532:c365556-365095 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI
Download Length: 462 bp
>T243111 NZ_OW969905:c42885-42601 [Citrobacter freundii]
GTGTATAAACTCACTGAACGGGCAGCAGACGACTTCGCCGGGATTTATGACTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACAGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGACTACCATA
CCGTTCCTGGTGTGATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTCTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA
GTGTATAAACTCACTGAACGGGCAGCAGACGACTTCGCCGGGATTTATGACTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACAGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGACTACCATA
CCGTTCCTGGTGTGATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTCTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA
Antitoxin
Download Length: 105 a.a. Molecular weight: 12063.56 Da Isoelectric Point: 6.4736
>AT243111 WP_000333630.1 NZ_CP096532:c365883-365569 [Staphylococcus aureus]
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYADYIRSKRK
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYADYIRSKRK
Download Length: 315 bp
>AT243111 NZ_OW969905:c43120-42878 [Citrobacter freundii]
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAACTCGATTCGTTTATTAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCGAGTGAAGTTATGCGTTCTGCGCTGCGATTGCTGGAGCAACAGGAAACAAACGATGAAGTTATTCGTCAGGCTG
TTATTGCTGGGCTGGAAAGCGGTGAAAGCTCGCTATCACTGCGGGATATTGCAGCACAAAGAAAACTGAGACATCGTGTA
TAA
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAACTCGATTCGTTTATTAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCGAGTGAAGTTATGCGTTCTGCGCTGCGATTGCTGGAGCAACAGGAAACAAACGATGAAGTTATTCGTCAGGCTG
TTATTGCTGGGCTGGAAAGCGGTGAAAGCTCGCTATCACTGCGGGATATTGCAGCACAAAGAAAACTGAGACATCGTGTA
TAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2I7Y5B3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E7YIA0 |