Detailed information of TA system TA243106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2162779..2163308
• Replicon: chromosome
• Accession: NZ_CP096530
• Organism: Staphylococcus aureus strain RIVM_M084526

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: M1F49_RS10785
• Protein ID: WP_000621175.1
• Coordinates: 2162779..2163141 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: M1F49_RS10790
• Protein ID: WP_000948331.1
• Coordinates: 2163138..2163308 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F49_RS10765 (M1F49_10760)	2159757..2160527	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
M1F49_RS10770 (M1F49_10765)	2160502..2160981	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
M1F49_RS10775 (M1F49_10770)	2160983..2161309	-	327	WP_001052491.1	anti-sigma factor antagonist	-
M1F49_RS10780 (M1F49_10775)	2161428..2162429	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
M1F49_RS10785 (M1F49_10780)	2162779..2163141	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M1F49_RS10790 (M1F49_10785)	2163138..2163308	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
M1F49_RS10795 (M1F49_10790)	2163393..2164541	-	1149	WP_001281139.1	alanine racemase	-
M1F49_RS10800 (M1F49_10795)	2164607..2164966	-	360	WP_000581197.1	holo-ACP synthase	-
M1F49_RS10805 (M1F49_10800)	2164970..2165461	-	492	WP_001286980.1	PH domain-containing protein	-
M1F49_RS10810 (M1F49_10805)	2165454..2167031	-	1578	WP_001294650.1	PH domain-containing protein	-
M1F49_RS10815 (M1F49_10810)	2167024..2167503	-	480	WP_001287081.1	hypothetical protein	-
M1F49_RS10820 (M1F49_10815)	2167712..2168272	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T243106 WP_000621175.1 NZ_CP096530:c2163141-2162779 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T243106 NZ_OW969904:3805500-3805718 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT243106 WP_000948331.1 NZ_CP096530:c2163308-2163138 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT243106 NZ_OW969904:3805098-3805472 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1