Detailed information of TA system TA243105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1995739..1996515
• Replicon: chromosome
• Accession: NZ_CP096530
• Organism: Staphylococcus aureus strain RIVM_M084526

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: M1F49_RS09835
• Protein ID: WP_000031108.1
• Coordinates: 1995739..1995891 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: M1F49_RS09840
• Protein ID: WP_001251224.1
• Coordinates: 1995916..1996515 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F49_RS09820 (M1F49_09815)	1991944..1992765	+	822	WP_000669380.1	RluA family pseudouridine synthase	-
M1F49_RS09825 (M1F49_09820)	1993220..1994605	-	1386	WP_000116238.1	class II fumarate hydratase	-
M1F49_RS09830 (M1F49_09825)	1994801..1995196	-	396	WP_000901018.1	hypothetical protein	-
M1F49_RS09835 (M1F49_09830)	1995739..1995891	-	153	WP_000031108.1	SAS053 family protein	Toxin
M1F49_RS09840 (M1F49_09835)	1995916..1996515	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
M1F49_RS09845 (M1F49_09840)	1996674..1997144	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
M1F49_RS09850 (M1F49_09845)	1997149..1998276	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
M1F49_RS09855 (M1F49_09850)	1998475..1999422	+	948	WP_014532515.1	IS30-like element ISSau1 family transposase	-
M1F49_RS09860 (M1F49_09855)	1999501..2000223	-	723	WP_031774878.1	amino acid ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1998475..1999422	947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T243105 WP_000031108.1 NZ_CP096530:c1995891-1995739 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T243105 NZ_OW969904:2479324-2479500 [Citrobacter freundii]
GTGAAGCAAAGTGAGTTTAGACGCTGGCTTGAATCTCAGGGAGTTGCTGTTACAAACGGCTCAAATCATCTGAAACTGAG
ATATCAGGGAAGGCGTAGCGTTATGCCCAGACATCCAGGTGATGAAATAAAAGAAGCCTTGCGAAAAGCGATTATTAAGC
AGCTTGACTTACAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT243105 WP_001251224.1 NZ_CP096530:c1996515-1995916 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT243105 NZ_OW969904:2479547-2479963 [Citrobacter freundii]
ATGCGTTATCCTGTTACTCTAACACCGGCAGTGGAAGGTGGGTTTGTGGTTTCTTTCCCGGATATTCCGGAAGCATTAAC
TCAGGGAAATACTCGCCATGACGCTTTGCAGGCCGCGCAGGCGGCATTGATTACCGCTTTTGAATTCTATTTCGACGACA
ACGAGGCGATTCCACTCCCTTCGGCGGTTAGCGCTGAAGATGACTACGTAGAAATACCGCTGAGCGTTGCGTCTAAGGTT
CTTCTGCTGAATGCCTTCCTTGAATCAAAAATCACCCAGCAGGAGCTGGCGAACAGGATCGGCAGACCTAAGCAGGAGAT
CACCCGTTTATTTGATTTAAAGCACACGACAAAAATTGATGCCGTGCAGATTGCTGCGCGCGCCTTAGGCAAAGAGCTGG
CGTTAACGATGTTGTAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4