Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /HTH_19(antitoxin)
Location 1625886..1626674 Replicon chromosome
Accession NZ_CP096530
Organism Staphylococcus aureus strain RIVM_M084526

Toxin (Protein)


Gene name - Uniprot ID A0A2I7Y5B3
Locus tag M1F49_RS07980 Protein ID WP_000525004.1
Coordinates 1626213..1626674 (+) Length 154 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0E7YIA0
Locus tag M1F49_RS07975 Protein ID WP_000333630.1
Coordinates 1625886..1626200 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1F49_RS07940 (M1F49_07935) 1621002..1621091 - 90 Protein_1559 hypothetical protein -
M1F49_RS07945 (M1F49_07940) 1621127..1622059 - 933 WP_001825312.1 DUF1672 domain-containing protein -
M1F49_RS07950 (M1F49_07945) 1622514..1623410 - 897 WP_001807840.1 DUF1672 domain-containing protein -
M1F49_RS07955 (M1F49_07950) 1623465..1624394 - 930 WP_001825313.1 DUF1672 domain-containing protein -
M1F49_RS07960 (M1F49_07955) 1624485..1624670 - 186 WP_000470336.1 hypothetical protein -
M1F49_RS07965 (M1F49_07960) 1624708..1625484 - 777 WP_001148544.1 Rha family transcriptional regulator -
M1F49_RS07970 (M1F49_07965) 1625498..1625734 - 237 WP_001121027.1 helix-turn-helix transcriptional regulator -
M1F49_RS07975 (M1F49_07970) 1625886..1626200 + 315 WP_000333630.1 helix-turn-helix transcriptional regulator Antitoxin
M1F49_RS07980 (M1F49_07975) 1626213..1626674 + 462 WP_000525004.1 hypothetical protein Toxin
M1F49_RS07985 (M1F49_07980) 1626692..1627276 + 585 WP_000825948.1 hypothetical protein -
M1F49_RS07990 (M1F49_07985) 1627506..1627652 + 147 WP_078067907.1 hypothetical protein -
M1F49_RS07995 (M1F49_07990) 1627649..1628263 - 615 WP_000191464.1 hypothetical protein -
M1F49_RS08000 (M1F49_07995) 1628389..1629594 + 1206 WP_031585161.1 tyrosine-type recombinase/integrase -
M1F49_RS08005 (M1F49_08000) 1629637..1631664 - 2028 WP_031914327.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(4-62)


Sequences

Toxin        


Download         Length: 154 a.a.        Molecular weight: 18074.46 Da        Isoelectric Point: 4.6915

>T243104 WP_000525004.1 NZ_CP096530:1626213-1626674 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Download         Length: 462 bp

>T243104 NZ_OW969904:2159965-2160068 [Citrobacter freundii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12063.56 Da        Isoelectric Point: 6.4736

>AT243104 WP_000333630.1 NZ_CP096530:1625886-1626200 [Staphylococcus aureus]
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYADYIRSKRK

Download         Length: 315 bp

>AT243104 NZ_OW969904:c2160073-2159930 [Citrobacter freundii]
CACATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I7Y5B3

Antitoxin

Source ID Structure
AlphaFold DB A0A0E7YIA0

References