Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2129810..2130324 Replicon chromosome
Accession NZ_CP096528
Organism Staphylococcus aureus strain RIVM_M084986

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag M1F50_RS10480 Protein ID WP_000581792.1
Coordinates 2129810..2130019 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag M1F50_RS10485 Protein ID WP_000138932.1
Coordinates 2130031..2130324 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1F50_RS10460 (M1F50_10440) 2125321..2126676 - 1356 WP_267439984.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
M1F50_RS10465 (M1F50_10445) 2126691..2127761 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
M1F50_RS10470 (M1F50_10450) 2128079..2129281 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
M1F50_RS10475 (M1F50_10455) 2129545..2129682 - 138 WP_000828354.1 Lmo0850 family protein -
M1F50_RS10480 (M1F50_10460) 2129810..2130019 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
M1F50_RS10485 (M1F50_10465) 2130031..2130324 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
M1F50_RS10490 (M1F50_10470) 2130490..2131974 + 1485 WP_000571550.1 cardiolipin synthase -
M1F50_RS10495 (M1F50_10475) 2132002..2132649 + 648 WP_001187614.1 HD domain-containing protein -
M1F50_RS10500 (M1F50_10480) 2133206..2133268 + 63 Protein_2025 hypothetical protein -
M1F50_RS10505 (M1F50_10485) 2133246..2134561 - 1316 Protein_2026 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T243096 WP_000581792.1 NZ_CP096528:c2130019-2129810 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T243096 NZ_OW969781:c4500443-4500186 [Klebsiella oxytoca]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCATATTCGCGATGCATC
AGGCCGCGTAACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAACTCATTAAGTCCGTCAACGGGCAGCCCTACCGTATCAATACCACCGGGCTGAACAACGTTCGCGCT
CAGCTGGACAACCGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT243096 WP_000138932.1 NZ_CP096528:c2130324-2130031 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT243096 NZ_OW969781:c4500175-4499624 [Klebsiella oxytoca]
ATGACAAACCATAACTTCACCTTTCACATCACCTCAGAGCGTGATGCTGATGATATTCGCGAAGTTGAAACCCGGGCCTT
CGGCTTTAGCAAAGAGGCCGATCTGGTTGCCGCTTTGCTCAACGATGAAAGTGCTCACTCGAGCTTATCTCTGCTGGCCA
AACATAATGGAAAAGCCGTTGGACATATTTTGTTCACCCTGGCGACGTTCAAAGGGGAGTCTGACTCTCCGATGATGCAT
ATCCTTGCCCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTGGGCGTAGGCGGATTGCTTATCCAGCGCGGTATTGAGCA
TCTGAAAGCCGCAGGAAGCGAGGCGGTATTTGTTCTGGGACATGCCACTTATTACCCGCGGCATGGCTTTGAACCCTGTG
CCGGAGATAAAGGCTATCCGGCGCCTTATCCCATTCCCGAGGAGCATAAAGCCTGCTGGATGCTGCAGCGGCTTTCGTCC
CGGCCGCTGGGGAGAACCGGTCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References