Detailed information of TA system TA243095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2110073..2110602
• Replicon: chromosome
• Accession: NZ_CP096528
• Organism: Staphylococcus aureus strain RIVM_M084986

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: M1F50_RS10390
• Protein ID: WP_000621175.1
• Coordinates: 2110073..2110435 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: M1F50_RS10395
• Protein ID: WP_000948331.1
• Coordinates: 2110432..2110602 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F50_RS10370 (M1F50_10350)	2107051..2107821	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
M1F50_RS10375 (M1F50_10355)	2107796..2108275	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
M1F50_RS10380 (M1F50_10360)	2108277..2108603	-	327	WP_001052491.1	anti-sigma factor antagonist	-
M1F50_RS10385 (M1F50_10365)	2108722..2109723	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
M1F50_RS10390 (M1F50_10370)	2110073..2110435	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M1F50_RS10395 (M1F50_10375)	2110432..2110602	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
M1F50_RS10400 (M1F50_10380)	2110687..2111835	-	1149	WP_001281139.1	alanine racemase	-
M1F50_RS10405 (M1F50_10385)	2111901..2112260	-	360	WP_000581197.1	holo-ACP synthase	-
M1F50_RS10410 (M1F50_10390)	2112264..2112755	-	492	WP_001286980.1	PH domain-containing protein	-
M1F50_RS10415 (M1F50_10395)	2112748..2114325	-	1578	WP_001294650.1	PH domain-containing protein	-
M1F50_RS10420 (M1F50_10400)	2114318..2114797	-	480	WP_001287081.1	hypothetical protein	-
M1F50_RS10425 (M1F50_10405)	2115006..2115566	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T243095 WP_000621175.1 NZ_CP096528:c2110435-2110073 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T243095 NZ_OW969781:4261237-4261455 [Klebsiella oxytoca]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGTTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCTGATAATGAACTGGCGGTATTTTACTCAGCAGCCGATCATCGTCTTGCTGAAC
TGACGATGAATAAATTGTATGACAAGATCCCGCCATCGGTCTGGAAATTCATCCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT243095 WP_000948331.1 NZ_CP096528:c2110602-2110432 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT243095 NZ_OW969781:4260836-4261210 [Klebsiella oxytoca]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTTAATGACCCAACCTCTGCTGTCAATCTTCAGCTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGGTGACTCAGCTTGACGAATATCTG
GATGACACTTTCGTGTTGTTCAGCAACTACGGCATTAACACTGCAGATCTGCAGAAATGGCGTAAATCAGGCAACAGGTT
ATTCCGCTGCTTTGTGAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1