Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 1948563..1949339 Replicon chromosome
Accession NZ_CP096528
Organism Staphylococcus aureus strain RIVM_M084986

Toxin (Protein)


Gene name tsbT Uniprot ID X5E2E6
Locus tag M1F50_RS09465 Protein ID WP_000031108.1
Coordinates 1948563..1948715 (-) Length 51 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID W8U4V4
Locus tag M1F50_RS09470 Protein ID WP_001251224.1
Coordinates 1948740..1949339 (-) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1F50_RS09450 (M1F50_09430) 1944768..1945589 + 822 WP_000669380.1 RluA family pseudouridine synthase -
M1F50_RS09455 (M1F50_09435) 1946044..1947429 - 1386 WP_000116238.1 class II fumarate hydratase -
M1F50_RS09460 (M1F50_09440) 1947625..1948020 - 396 WP_000901018.1 hypothetical protein -
M1F50_RS09465 (M1F50_09445) 1948563..1948715 - 153 WP_000031108.1 SAS053 family protein Toxin
M1F50_RS09470 (M1F50_09450) 1948740..1949339 - 600 WP_001251224.1 glucosamine-6-phosphate isomerase Antitoxin
M1F50_RS09475 (M1F50_09455) 1949498..1949968 - 471 WP_000181394.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
M1F50_RS09480 (M1F50_09460) 1949973..1951100 - 1128 WP_000379980.1 tRNA epoxyqueuosine(34) reductase QueG -
M1F50_RS09485 (M1F50_09465) 1951251..1951973 - 723 WP_031774878.1 amino acid ABC transporter ATP-binding protein -
M1F50_RS09490 (M1F50_09470) 1951966..1953423 - 1458 WP_031776111.1 ABC transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(124-196)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5936.28 Da        Isoelectric Point: 3.8962

>T243094 WP_000031108.1 NZ_CP096528:c1948715-1948563 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Download         Length: 153 bp

>T243094 NZ_OW969781:2090870-2090973 [Klebsiella oxytoca]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTTTTTTT

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22343.47 Da        Isoelectric Point: 5.1445

>AT243094 WP_001251224.1 NZ_CP096528:c1949339-1948740 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Download         Length: 600 bp

>AT243094 NZ_OW969781:c2090980-2090835 [Klebsiella oxytoca]
AAAAGATAAAAAAAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X5E2E6

Antitoxin

Source ID Structure
AlphaFold DB W8U4V4

References