Detailed information of TA system TA243093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 913799..914275
• Replicon: chromosome
• Accession: NZ_CP096528
• Organism: Staphylococcus aureus strain RIVM_M084986

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: M1F50_RS04490
• Protein ID: WP_000052076.1
• Coordinates: 913799..913987 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: M1F50_RS04495
• Protein ID: WP_001059690.1
• Coordinates: 914015..914275 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F50_RS04460 (M1F50_04445)	909828..910310	+	483	WP_000824489.1	hypothetical protein	-
M1F50_RS04465 (M1F50_04450)	910651..911337	+	687	WP_001574556.1	thermonuclease family protein	-
M1F50_RS04470 (M1F50_04455)	911694..911894	+	201	WP_001059082.1	cold-shock protein	-
M1F50_RS04475 (M1F50_04460)	912391..912609	-	219	WP_000005881.1	hypothetical protein	-
M1F50_RS04480 (M1F50_04465)	912670..912954	-	285	WP_001004403.1	hypothetical protein	-
M1F50_RS04485 (M1F50_04470)	913042..913611	-	570	WP_001106655.1	lipoprotein N-acylation protein LnsA	-
M1F50_RS04490 (M1F50_04475)	913799..913987	-	189	WP_000052076.1	hypothetical protein	Toxin
M1F50_RS04495 (M1F50_04480)	914015..914275	-	261	WP_001059690.1	hypothetical protein	Antitoxin
M1F50_RS04500 (M1F50_04485)	914617..914820	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
M1F50_RS04505 (M1F50_04490)	914817..915053	-	237	WP_000368687.1	hypothetical protein	-
M1F50_RS04510 (M1F50_04495)	915278..915859	+	582	WP_001140696.1	histidine phosphatase family protein	-
M1F50_RS04515 (M1F50_04500)	915932..916549	-	618	WP_000255562.1	LysE/ArgO family amino acid transporter	-
M1F50_RS04520 (M1F50_04505)	916698..917192	-	495	WP_000569881.1	GNAT family N-acetyltransferase	-
M1F50_RS04525 (M1F50_04510)	917591..918013	-	423	WP_000974452.1	organic hydroperoxide resistance protein	-
M1F50_RS04530 (M1F50_04515)	918161..918877	+	717	WP_000150026.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T243093 WP_000052076.1 NZ_CP096528:c913987-913799 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T243093 NZ_OW969781:873806-874216 [Klebsiella oxytoca]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAATGGTTCTCACTGTTGATGCACGGCGTGGTGGCGGC
GTTGGTGCTGGTGTTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCAGACAGGGGGAAATAAAGCTGCTGATTGACTCTCGCCTGCGCTGGCAAAAAGCG
GAGTGGGATATCGTCGGCACCCCGTGGGTAATCAATAGCGGAATGCTGCTGCGCCTGCGAAATACGGAAAACCAACGCAC
TCAGCATCTATGGGTGGCGGCGGATAGCATGGACGCGGGCGAGTGGCGCGATCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGATTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT243093 WP_001059690.1 NZ_CP096528:c914275-914015 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT243093 NZ_OW969781:873559-873825 [Klebsiella oxytoca]
ATGGATATTAACAATAAAGCTCGCATCCACTGGGCATGCCGCCGGGGAATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTTGAGCATGAGTACGATACGCTCAGTGATGACGATAAGCGGTTGTTCATCCGCCTGCTGGAAAATGACGATCCGGACC
TGTTCAACTGGTTGATGAACCATGGCGAACCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56